IGEM:Peking University/2008/Experiment/PKU 080519 G1 1: Difference between revisions
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|BamHI||0.2|| | |BamHI||0.2|| | ||
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| | |K Buffer||2 | ||
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|ddH<sub>2</sub>O||13.6 | |ddH<sub>2</sub>O||13.6 | ||
Latest revision as of 21:47, 11 July 2008
PKU_080519_G1_1
- Cultivation- E.Coli(liguid-Amp+)
- Plasmid Miniprep-pGREG504/pADH
- Enzyme cutting-pGREG504/pADH-EcoRV
| Volume (μL) | ||
| pGREG504/pADH | 5 | |
| EcoRV | 0.2 | *13 |
| H Buffer | 2 | |
| ddH2O | 12.8 |
- Enzyme cutting-pGREG504/pADH-SacI-BamHI
| Volume (μL) | ||
| pGREG504/pADH | 5 | |
| SacI | 0.2 | *13 |
| BamHI | 0.2 | |
| K Buffer | 2 | |
| ddH2O | 13.6 |
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