IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
::'''Enzyme cutting-pGREG504/pADH-EcoRV'''
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[[Image:PKU_080519_check-pGREG504-pADH.tif|400px]]
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[[Image:PKU_080519_check-pGREG504-pADH.tif|600px]]
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Revision as of 13:06, 3 July 2008

PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pGREG504/pADH
Enzyme cutting-pGREG504/pADH-EcoRV
Volume (μL)
pGREG504/pADH5
EcoRV0.2*13
H Buffer2
ddH2O12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH5
SacI0.2*13
BamHI0.2
H Buffer2
ddH2O13.6


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