IGEM:Peking University/2008/Experiment/PKU 080519 G1 1

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PKU_080519_G1_1

Cultivation- E.Coli(liguid-Amp⁺)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV


	Volume (μL)
pGREG504/pADH	5
EcoRV	0.2	*13
H Buffer	2
ddH₂O	12.8

Enzyme cutting-pGREG504/pADH-SacI-BamHI


	Volume (μL)
pGREG504/pADH	5
SacI	0.2	*13
BamHI	0.2
H Buffer	2
ddH₂O	13.6

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