IGEM:Peking University/2008/Experiment/PKU 080527 G1 1: Difference between revisions
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(New page: ==PKU_080527_G1_1== <hr class=divider> ::'''PCR- pGREG504/(pESC-hAID)-hAID-50ul''' {|border="1" cellspacing="0" |+ |- | ||Volume (μL) |- | pGREG504/(pESC-hAID)||1 |- | transtart taq|...) |
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| Line 12: | Line 12: | ||
| transtart taq||1 | | transtart taq||1 | ||
|- | |- | ||
| transtart buffer|5 | | transtart buffer||5 | ||
|- | |- | ||
| dNTP||3 | | dNTP||3 | ||
| Line 40: | Line 40: | ||
| 56||02:00||30 cycles | | 56||02:00||30 cycles | ||
|- | |- | ||
| 72||01:00||cycles | | 72||01:00||30 cycles | ||
|- | |- | ||
| 72||05:00 | | 72||05:00 | ||
|} | |} | ||
<hr class=divider> | |||
::'''Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
| pGREG506'/(pADH-lacO1)||5 | |||
|- | |||
|ClaI||0.2 | |||
|- | |||
|BamHI||0.2 | |||
|- | |||
|K Buffer||2 | |||
|- | |||
|ddH<sub>2</sub>O||12.6 | |||
|} | |||
didn't work | |||
{|align="right" | {|align="right" | ||
|back to [[PKU_Experiment|Previous Page]] | |back to [[PKU_Experiment|Previous Page]] | ||
|} | |} | ||
Revision as of 03:54, 4 July 2008
PKU_080527_G1_1
- PCR- pGREG504/(pESC-hAID)-hAID-50ul
| Volume (μL) | |
| pGREG504/(pESC-hAID) | 1 |
| transtart taq | 1 |
| transtart buffer | 5 |
| dNTP | 3 |
| F-Primer | 1 |
| R-Primer | 1 |
| ddH2O | 58 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 04:00 | |
| 50 | 02:00 | |
| 72 | 02:00 | |
| 94 | 01:00 | 30 cycles |
| 56 | 02:00 | 30 cycles |
| 72 | 01:00 | 30 cycles |
| 72 | 05:00 |
- Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
| Volume (μL) | |
| pGREG506'/(pADH-lacO1) | 5 |
| ClaI | 0.2 |
| BamHI | 0.2 |
| K Buffer | 2 |
| ddH2O | 12.6 |
didn't work
| back to Previous Page |
