IGEM:Peking University/2008/Experiment/PKU 080527 G1 1
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PKU_080527_G1_1
- PCR- pGREG504/(pESC-hAID)-hAID-50ul
| Volume (μL) | |
| pGREG504/(pESC-hAID) | 1 |
| transtart taq | 1 |
| 5 | |
| dNTP | 3 |
| F-Primer | 1 |
| R-Primer | 1 |
| ddH2O | 58 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 04:00 | |
| 50 | 02:00 | |
| 72 | 02:00 | |
| 94 | 01:00 | 30 cycles |
| 56 | 02:00 | 30 cycles |
| 72 | 01:00 | cycles |
| 72 | 05:00 |
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