IGEM:Peking University/2008/Experiment/PKU 080527 G1 1

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PKU_080527_G1_1


PCR- pGREG504/(pESC-hAID)-hAID-50ul
Volume (μL)
pGREG504/(pESC-hAID)1
transtart taq1
transtart buffer5
dNTP3
F-Primer1
R-Primer1
ddH2O58


conditions
Temperature(°C)Time Length(0:00:00)
9404:00
5002:00
7202:00
9401:0030 cycles
5602:0030 cycles
7201:0030 cycles
7205:00

Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
Volume (μL)
pGREG506'/(pADH-lacO1)5
ClaI0.2
BamHI0.2
K Buffer2
ddH2O12.6

didn't work

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