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PKU_080527_G1_1
- PCR- pGREG504/(pESC-hAID)-hAID-50ul
|
Volume (μL)
|
pGREG504/(pESC-hAID) |
1
|
transtart taq |
1
|
transtart buffer |
5
|
dNTP |
3
|
F-Primer |
1
|
R-Primer |
1
|
ddH2O |
58
|
- conditions
Temperature(°C) |
Time Length(0:00:00)
|
94 |
04:00
|
50 |
02:00
|
72 |
02:00
|
94 |
01:00 |
30 cycles
|
56 |
02:00 |
30 cycles
|
72 |
01:00 |
30 cycles
|
72 |
05:00
|
- Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
|
Volume (μL)
|
pGREG506'/(pADH-lacO1) |
5
|
ClaI |
0.2
|
BamHI |
0.2
|
K Buffer |
2
|
ddH2O |
12.6
|
didn't work