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PKU_080527_G1_1
- PCR- pGREG504/(pESC-hAID)-hAID-50ul
|
Volume (μL)
|
| pGREG504/(pESC-hAID) |
1
|
| transtart taq |
1
|
| transtart buffer |
5
|
| dNTP |
3
|
| F-Primer |
1
|
| R-Primer |
1
|
| ddH2O |
58
|
- conditions
| Temperature(°C) |
Time Length(0:00:00)
|
| 94 |
04:00
|
| 50 |
02:00
|
| 72 |
02:00
|
| 94 |
01:00 |
30 cycles
|
| 56 |
02:00 |
30 cycles
|
| 72 |
01:00 |
30 cycles
|
| 72 |
05:00
|
- Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI
|
Volume (μL)
|
| pGREG506'/(pADH-lacO1) |
5
|
| ClaI |
0.2
|
| BamHI |
0.2
|
| K Buffer |
2
|
| ddH2O |
12.6
|
didn't work
