IGEM:Peking University/2008/Experiment/PKU 0807017 G1 1
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Wenjun Shao
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PKU_080717_G1_1
PCR- pGREG504-pADH-(lac1/lac2/lacS/tet)-50ul
Using program lac12MQT
Enzyme cutting-pADH-(lac1/lac2/lacS/tet)(PCR product)
Volume (μL)
Promoter
15
BamHI
2
SacI
2
0.5×K Buffer
5
ddH
2
O
76
Volume (μL)
Promoter
15
SacI
5
XhoI
5
1×M Buffer
10
ddH
2
O
65
Enzyme cutting-AID
Volume (μL)
AID
15
BamHI
2
XhoI
2
1×K Buffer
10
ddH
2
O
71
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