IGEM:Peking University/2008/Experiment/PKU 080708 G1 1: Difference between revisions

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(New page: ==PKU_080708_G1_1== <hr class=divider> <hr class=divider> ::'''Cultivation- E.Coli(liguid-Amp<sup>+</sup>)''' <hr class=divider> ::'''Plasmid Miniprep-pADH-lac1-T''' <hr class=divider> ...)
 
 
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::'''Gel purification'''
::'''Gel running'''




Latest revision as of 08:45, 13 July 2008

PKU_080708_G1_1

Cultivation- E.Coli(liguid-Amp+)
Plasmid Miniprep-pADH-lac1-T
Enzyme cutting-pADH-lac1-T
Volume (μL)
pADH-lac1-T 15
SacI 5 *2
XhoI 5
M Buffer 10
ddH2O 65


Volume (μL)
pADH-lac1-T 15
SacI 2 *2
XhoI 2
HindIII 2
M Buffer 10
ddH2O 69


Enzyme cutting-pADH-lac?-AIDwrong
Volume (μL)
pADH-lac?-AIDwrong 10
BamHI 2 *3
XhoI 2
K Buffer 10
ddH2O 76


Enzyme cutting-pGREG504
Volume (μL)
pGREG504 10
SacI 5
XhoI 5
M Buffer 10
ddH2O 70


Gel running


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