IGEM:Peking University/2008/Experiment/PKU 080709 G1 1: Difference between revisions
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Wenjun Shao (talk | contribs) (New page: ==PKU_080709_G1_1== <hr class=divider> ::'''Gel purification-pADH-lac1-T''' <hr class=divider> ::'''PCR-lac2/lac1/tet/lacS''' {|border="1" cellspacing="0" align="center" |+ |- | ||Vol...) |
Wenjun Shao (talk | contribs) |
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\\**lac2 uses lac2 primer; others use lac1 primer | \\**lac2 uses lac2 primer; others use lac1 primer | ||
::'''Conditions''' | |||
::''' | |||
Revision as of 09:00, 13 July 2008
PKU_080709_G1_1
- Gel purification-pADH-lac1-T
- PCR-lac2/lac1/tet/lacS
| Volume (μL) | |
| Template* | 5 |
| dNTP | 5 |
| KOD Plus Transtart Buffer | 5 |
| Transtart | |
| F-Primer | 1 |
| R-Primer | 1 |
| ddH2O | 36 |
\*"Template" stands for lac2, lac1, tet, lacS each \\**lac2 uses lac2 primer; others use lac1 primer
- Conditions
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