IGEM:Peking University/2008/Notebook/Group 1/2008/05/27: Difference between revisions
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(Autocreate 2008/05/27 Entry for IGEM:Peking_University/2008/Notebook/Group_1) |
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== | ==PKU_080527_G1_1== | ||
<hr class=divider> | |||
::'''PCR- pGREG504/(pESC-hAID)-hAID-50ul''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
| pGREG504/(pESC-hAID)||1 | |||
|- | |||
| transtart taq||1 | |||
|- | |||
| transtart buffer||5 | |||
|- | |||
| dNTP||3 | |||
|- | |||
|F-Primer||1 | |||
|- | |||
|R-Primer||1 | |||
|- | |||
|ddH<sub>2</sub>O||58 | |||
|} | |||
::'''conditions''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
|Temperature(°C)||Time Length(0:00:00) | |||
|- | |||
| 94||04:00 | |||
|- | |||
| 50||02:00 | |||
|- | |||
| 72||02:00 | |||
|- | |||
| 94||01:00||30 cycles | |||
|- | |||
| 56||02:00||30 cycles | |||
|- | |||
| 72||01:00||30 cycles | |||
|- | |||
| 72||05:00 | |||
|} | |||
<hr class=divider> | |||
::'''Enzyme cutting-pGREG506'/(pADH-lacO1)-ClaI-BamHI''' | |||
{|border="1" cellspacing="0" | |||
|+ | |||
|- | |||
| ||Volume (μL) | |||
|- | |||
| pGREG506'/(pADH-lacO1)||5 | |||
|- | |||
|ClaI||0.2 | |||
|- | |||
|BamHI||0.2 | |||
|- | |||
|K Buffer||2 | |||
|- | |||
|ddH<sub>2</sub>O||12.6 | |||
|} | |||
didn't work | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> |
Revision as of 08:28, 18 July 2008
Group 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||
PKU_080527_G1_1
didn't work |