IGEM:Peking University/2008/Notebook/Group 1/2008/07/19

From OpenWetWare
Jump to navigationJump to search
Group 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PKU_080719_G1_1

Transformation-single/double ligation-pGREG504-pAID-lac2/Control(C1~C5)


Gel running-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)


Gel extraction-enzyme cutting product-pADH-lac1/lacS/tet(single/double ligation)
Changing kit (QIAquick)
Gel running-check extraction product
lanes:1BS|SBS|TBS|AII|PGSX|1SX|SSX|TSX


Single Ligation-pGREG504-pAID-lac1/S/tet
Volume (μL)
promoter 4
pGREG504 1
ligation buffer 5


Double Ligation-pGREG504-pAID-lac1/S/tet-AID
Volume (μL)
promoter 3
AID 3
pGREG504 1
ligation buffer 7


Control C2-1
Volume (μL)
promoter(single ligation) 4
ddH2O 1
ligation buffer 5


Control C2-2
Volume (μL)
promoter(double ligation) 3
ddH2O 4
ligation buffer 7


Control C2-3
Volume (μL)
promoter(double ligation) 4
AID 3
ddH2O 1
ligation buffer 7


Control C2-4
Volume (μL)
ddH2O 5
ligation buffer 5



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Notebook/Group_1/2008/07/19&oldid=223213"