IGEM:Peking University/2008/Notebook/Group 1/2008/07/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 61: Line 61:


'''Conlusion:'''
'''Conlusion:'''
1. The plasmids are right from the enzyme cutting result: only 700bp bands, no 1.4kb bands, showing the exact fragment we need.
1. The plasmids are right from the enzyme cutting result: only 700bp bands, no 1.4kb bands, showing the exact fragment we need.
2. The middle one looks nicer, which suggests the following aspects MAY be reasons: a, shorter enzyme cutting time (since BamHI has potential spark activity); b, higher gel running volt (time to run gel becomes shorter); c, new K buffer (other materials are totally the same, including the enzyme containers).
2. The middle one looks nicer, which suggests the following aspects MAY be reasons: a, shorter enzyme cutting time (since BamHI has potential spark activity); b, higher gel running volt (time to run gel becomes shorter); c, new K buffer (other materials are totally the same, including the enzyme containers).


Revision as of 10:28, 28 July 2008

Group 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PKU_080728_G1_1

This part will be updated soon


Testing result-Xili's plasmid-pADH-lac1/2/S/tet


Digestion-pADH-lac1/2-1/2-2/S/tet-ClaI-BamHI


Gel running and testing


Gel Picture: 080725-xili_plasmid_test-redigest

lanes:1kb marker, 100bp marker, lac1, lac2-1, lac2-2, lacS, tet, lambda marker


Transformation-xili-lac1-1/1-2/2-1/2-2/S-1/S-2/tet-1/tet-2(from two envelops)


Picking up colonies|Miniprep|digestion(40min)|Gel running(140V)


Gel Picture: 080728-xili_plasmid_test-redigest

Lanes: lambda marker, {lac2-2-1, lac2-2-2, lac2-2-3, lac2-2-4, lac2-2-5, lac2-2-6, lacS-2-1}0727miniprep, {lac1-1-1, lac2-2-1, lac2-2-2, lac2-2-3, lacS-1-1, lacS-2-1}0726miniprep, 100bp marker


Picking up colonies|Miniprep|digestion(90min)|Gel running(120V)


Gel Picture: 080728-xili_plasmid_test-redigest-longer_time

Lanes: {lac2-2-1, lac2-2-2, lac2-2-3, lac2-2-4, lac2-2-5, lac2-2-6, lacS-2-1}0727miniprep, lambda marker, {lac1-1-1, lac2-2-1, lac2-2-2, lac2-2-3, lacS-1-1, lacS-2-1}0726miniprep


Conlusion:

1. The plasmids are right from the enzyme cutting result: only 700bp bands, no 1.4kb bands, showing the exact fragment we need.

2. The middle one looks nicer, which suggests the following aspects MAY be reasons: a, shorter enzyme cutting time (since BamHI has potential spark activity); b, higher gel running volt (time to run gel becomes shorter); c, new K buffer (other materials are totally the same, including the enzyme containers).



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Notebook/Group_1/2008/07/28&oldid=225088"