IGEM:Peking University/2008/Notebook/Group 1/2008/07/28: Difference between revisions
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===Report:Testing result-Xili's plasmid-pADH-lac1/2/S/tet=== | ===Report: Testing result-Xili's plasmid-pADH-lac1/2/S/tet=== | ||
Revision as of 10:30, 28 July 2008
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PKU_080728_G1_1This part will be updated soon
Report: Testing result-Xili's plasmid-pADH-lac1/2/S/tetDigestion-pADH-lac1/2-1/2-2/S/tet-ClaI-BamHI
lanes:1kb marker, 100bp marker, lac1, lac2-1, lac2-2, lacS, tet, lambda marker
Lanes: lambda marker, {lac2-2-1, lac2-2-2, lac2-2-3, lac2-2-4, lac2-2-5, lac2-2-6, lacS-2-1}0727miniprep, {lac1-1-1, lac2-2-1, lac2-2-2, lac2-2-3, lacS-1-1, lacS-2-1}0726miniprep, 100bp marker
Lanes: {lac2-2-1, lac2-2-2, lac2-2-3, lac2-2-4, lac2-2-5, lac2-2-6, lacS-2-1}0727miniprep, lambda marker, {lac1-1-1, lac2-2-1, lac2-2-2, lac2-2-3, lacS-1-1, lacS-2-1}0726miniprep
1. The plasmids are right from the enzyme cutting result: only 700bp bands, no 1.4kb bands, showing the exact fragment we need. 2. The middle one looks nicer--nearly no smearing phenomena, which suggests the following aspects MAY be reasons: a, shorter enzyme cutting time (since BamHI has potential spark activity); b, higher gel running volt (time to run gel becomes shorter); c, new K buffer (other materials are totally the same, including the enzyme containers).
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