IGEM:Peking University/2008/Notebook/Group 2/2008/06/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2008/06/03 Entry for IGEM:Peking_University/2008/Notebook/Group_2)
 
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
==Entry title==
==PCR==
* Insert your content here.
{|border="1" cellspacing="0"
|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq  20ul*6'''
|-
| ||Volume (μL)
|-
|Template （Budding Yeast Genome）||1
|-
|dNTP||2
|-
|Enzyme||0.5
|-
|Buffer||2
|-
|F-Gal4-HindIII-ApaI ||0.5
|-
|R-Gal4-EcoRI-Xhol||0.5
|-
|ddH2O||13.5
|}


::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature(&&&)||Time Length(0:00:00)
|-
| 94°C||02:00
|-
| 94°C||01:00||4 cycles
|-
| 50°C||02:00||4 cycles
|-
| 72°C||00:30||4 cycles
|-
| 94°C||01:00||30 cycles
|-
| 52.0,53.6,56.1,58.8,60.1,62.0°C||02:00||30 cycles
|-
| 72°C||04:00||30 cycles
|-
| 72°C||05:00
|-
| 4°C||Hold
|}
<hr class=divider>
<br>
{|border="1" cellspacing="0"
|+ '''TITLE: PCR-Budding Yeast Genome-Gal4-Transtart  50ul*3'''
|-
| ||Volume (μL)
|-
|Template （Budding Yeast Genome）||1
|-
|dNTP||5
|-
|Enzyme||1
|-
|Buffer||5
|-
|F-Gal4-HindIII-ApaI ||1
|-
|R-Gal4-EcoRI-Xhol||1
|-
|ddH2O||36
|}
::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature||Time Length(0:00:00)
|-
| 94°C||02:00
|-
| 94°C||01:00||4 cycles
|-
| 50°C||02:00||4 cycles
|-
| 72°C||00:30||4 cycles
|-
| 94°C||01:00||30 cycles
|-
| 54.7,57.4,60.1°C||02:00||30 cycles
|-
| 72°C||04:00||30 cycles
|-
| 72°C||05:00
|-
| 4°C||Hold
|}




Revision as of 12:16, 22 July 2008

Group 2 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PCR

TITLE: PCR-Budding Yeast Genome-Gal4-Easytaq 20ul*6
Volume (μL)
Template （Budding Yeast Genome） 1
dNTP 2
Enzyme 0.5
Buffer 2
F-Gal4-HindIII-ApaI 0.5
R-Gal4-EcoRI-Xhol 0.5
ddH2O 13.5
conditions
Temperature(&&&) Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 00:30 4 cycles
94°C 01:00 30 cycles
52.0,53.6,56.1,58.8,60.1,62.0°C 02:00 30 cycles
72°C 04:00 30 cycles
72°C 05:00
4°C Hold


TITLE: PCR-Budding Yeast Genome-Gal4-Transtart 50ul*3
Volume (μL)
Template （Budding Yeast Genome） 1
dNTP 5
Enzyme 1
Buffer 5
F-Gal4-HindIII-ApaI 1
R-Gal4-EcoRI-Xhol 1
ddH2O 36
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 00:30 4 cycles
94°C 01:00 30 cycles
54.7,57.4,60.1°C 02:00 30 cycles
72°C 04:00 30 cycles
72°C 05:00
4°C Hold



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Notebook/Group_2/2008/06/03&oldid=223240"