IGEM:Peking University/2008/Notebook/Group 2/2008/06/23: Difference between revisions

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(Autocreate 2008/06/23 Entry for IGEM:Peking_University/2008/Notebook/Group_2)
 
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==Entry title==
==0623==
* Insert your content here.
{|border="1" cellspacing="0"
 
|+ '''TITLE: PCR-pPT-Gal4-Transtart  50ul*2'''
|-
| ||Volume (μL)
|-
|Template||1
|-
|dNTP||5
|-
|Enzyme||0.5
|-
|Buffer||5
|-
|F-Primer||0.5
|-
|R-Primer||0.5
|-
|ddH<sub>2O</sub>||37.5
|}


::'''conditions'''
{|border="1" cellspacing="0"
|+
|-
|Temperature||Time Length(0:00:00)
|-
| 94°C||02:00
|-
| 94°C||01:00||4 cycles
|-
| 50°C||02:00||4 cycles
|-
| 72°C||05:00||4 cycles
|-
| 94°C||01:00||30 cycles
|-
| 57.4,58.8°C||01:00||30 cycles
|-
| 72°C||03:00||30 cycles
|-
| 72°C||10:00
|-
| 4°C||Hold
|}


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Revision as of 12:24, 22 July 2008

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0623

TITLE: PCR-pPT-Gal4-Transtart 50ul*2
Volume (μL)
Template 1
dNTP 5
Enzyme 0.5
Buffer 5
F-Primer 0.5
R-Primer 0.5
ddH2O 37.5
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
57.4,58.8°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold


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