IGEM:Peking University/2008/Notebook/Group 2/2008/07/25

From OpenWetWare
Jump to navigationJump to search
Group 2 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

PKU_080725_G2_1

Frame Shifting mutation-513C514-50μL system-primers 0.75μL each


Digestion-1.4μL DpnI-37°C-2h


Self circling



Enzyme cutting-Gal4wt-ApaI-SpeI


Gel extraction


Enzyme cutting-pGREG505-lexO-ApaI-SpeI


Ethanol precipitation


Ligation-Gal4wt-pGREG505-lexO


Gel running



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking_University/2008/Notebook/Group_2/2008/07/25&oldid=224677"