IGEM:PennState/2006/Eikenplates

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===<font color="red">Procedure</font>===
===<font color="red">Procedure</font>===
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1. Add the following ingredients:
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# Add the following ingredients:
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#*0.25g tryptone
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*0.25g tryptone
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#*0.20g NaCl
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*0.20g NaCl
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#*0.05g eiken agar
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*0.05 eiken agar
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#*0.15g sodium citrate
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*0.15g sodium citrate
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#*25ml water
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*25ml water
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# Autoclave 20 min slow exhaust.
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# Add appropriate antibiotic
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# This gives a final [eiken] of 0.20%
 +
 
 +
*Alternatively create a .28% Eiken Agar plate using ~.07g eiken agar
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2. Autoclave 20 min slow exhaust.
 
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3. Add appropriate antibiotic
 
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4. This gives a final [eiken] of 0.20%
 
[http://openwetware.org/wiki/IGEM:PennState Main]
[http://openwetware.org/wiki/IGEM:PennState Main]

Revision as of 09:57, 5 July 2007

Eiken Agar Plates

Eiken plates should be made up the same day or no more than one day before using them. Otherwise they dry out and cells do not swarm.

Procedure

  1. Add the following ingredients:
    • 0.25g tryptone
    • 0.20g NaCl
    • 0.05g eiken agar
    • 0.15g sodium citrate
    • 25ml water
  2. Autoclave 20 min slow exhaust.
  3. Add appropriate antibiotic
  4. This gives a final [eiken] of 0.20%
  • Alternatively create a .28% Eiken Agar plate using ~.07g eiken agar



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