IGEM:PennState/2006/Eikenplates

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Current revision (15:22, 10 July 2007) (view source)
 
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Eiken plates should be made up the same day or no more than one day before using them.  Otherwise they dry out and cells do not swarm.  
Eiken plates should be made up the same day or no more than one day before using them.  Otherwise they dry out and cells do not swarm.  
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===<font color="red">Procedure (regular 10cm plates)(</font>===
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===<font color="red">Procedure (regular 10cm plates)</font>===
# Add the following ingredients:
# Add the following ingredients:

Current revision

Eiken Agar Plates

Eiken plates should be made up the same day or no more than one day before using them. Otherwise they dry out and cells do not swarm.

Procedure (regular 10cm plates)

  1. Add the following ingredients:
    • 0.25g tryptone
    • 0.20g NaCl
    • 0.05g eiken agar
    • 0.15g sodium citrate
    • 25ml water
  2. Autoclave 20 min slow exhaust.
  3. Add appropriate antibiotic
  4. This gives a final [eiken] of 0.20%
  • Alternatively create a .28% Eiken Agar plate using ~.07g eiken agar


Procedure (big 15cm plates)

  1. Add the following ingredients:
    • 0.56g tryptone
    • 0.45g NaCl
    • 0.11g eiken agar
    • 0.338g sodium citrate
  2. 56.25 ml water
  3. Autoclave 20 min slow exhaust.
  4. Add appropriate antibiotic
  5. gives a final [eiken] of 0.20%
  • This Alternatively create a .28% Eiken Agar plate using ~.1575g eiken agar


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