IGEM:PennState/2006/LBPlates: Difference between revisions

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='''LB Plates'''=


===<font color="red">LB Plates</font>===
(per/L H2O)
1. Weigh and add to H2O in large Erlenmeyer:
*10 g Bacto-tryptone
*5 g Bacto-yeast extract
*10 g NaCl
*15 g Bacto-agar
2. Autoclave on slow exhaust cycle 25 min
3. Cool in tap-water-bath 10 min, then place in 45ºC water bath.
(in sterile hood)
4. When media has cooled to ~45ºC (comfortable to hold), add appropriate antibiotic. 
5. Pour plates (~25 mL/plate)
6. Allow to solidify & store in bag in cold-room
[http://openwetware.org/wiki/IGEM:PennState Main]

Latest revision as of 12:28, 8 August 2007