IGEM:PennState/2006/Microchannel

From OpenWetWare

< IGEM:PennState | 2006
Revision as of 14:00, 27 October 2006 by Luw134 (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Polydimethylsiloxane (PDMS) Prep

  • 1. Add 10g PDMS and 1g curing agent to a weigh boat
  • 2. Stir vigorously for 3 to 5 minutes (the mixture should be opaque from trapped gas bubbles)
  • 3. Allow the mixture to set for 20-30 minutes or until all bubbles are gone


Spin Coating

  • 1. Prepare PDMS as shown above no more than 1 hour before you're ready to spin coat
  • 2. Attach the selected mold to the spin coating machine
  • 3. Pour PDMS slowly and evenly over the entire mold
  • 4. Spin the mold for 30 seconds at 500rpm
  • 5. Carefully remove the coated mold from the spin coating machine and place it a leveled oven to cure for 2 hours at 75C
  • 6. Using a razor, cut out the desired pieces of the micro channel array and place them in a Petri dish (make sure to note the orientation of the channels for storage)


PDMS Surface Treatment via O2 Plasma

  • 1. Place the PDMS channels "face up" on the O2 Plasma device platform
  • 2. Run the coating procedure for 1 minute
  • 3. Immediately transfer the treated channels to agar media (treated micro channels should be face down to maintain their hydrophilic state)


Main

Personal tools