http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&feed=atom&action=historyIGEM:PennState/2006/Progress motility - Revision history2013-05-23T18:32:39ZRevision history for this page on the wikiMediaWiki 1.13.2http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82660&oldid=prevLuw134 at 03:34, 28 October 20062006-10-28T03:34:58Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:34, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|center<del class="diffchange diffchange-inline">|thumb|</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|center]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.'''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82659&oldid=prevLuw134 at 03:34, 28 October 20062006-10-28T03:34:47Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:34, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|center|thumb|<del class="diffchange diffchange-inline">'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|center|thumb|]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.'''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82658&oldid=prevLuw134 at 03:34, 28 October 20062006-10-28T03:34:18Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:34, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|<del class="diffchange diffchange-inline">top|left</del>|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|<ins class="diffchange diffchange-inline">center</ins>|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82657&oldid=prevLuw134 at 03:34, 28 October 20062006-10-28T03:34:04Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:34, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg<ins class="diffchange diffchange-inline">|top</ins>|left|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= =</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82656&oldid=prevLuw134 at 03:33, 28 October 20062006-10-28T03:33:52Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:33, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">= =</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82655&oldid=prevLuw134 at 03:33, 28 October 20062006-10-28T03:33:43Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:33, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|thumb|<del class="diffchange diffchange-inline">FIG 6. </del>'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|thumb|'''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82654&oldid=prevLuw134 at 03:33, 28 October 20062006-10-28T03:33:19Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:33, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|FIG 6. '''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates. <del class="diffchange diffchange-inline"> </del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left<ins class="diffchange diffchange-inline">|thumb</ins>|FIG 6. '''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82653&oldid=prevLuw134 at 03:32, 28 October 20062006-10-28T03:32:37Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:32, 28 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|<ins class="diffchange diffchange-inline">FIG 6. '''Swarm plate assay of Constructs C, D, and E with and without IPTG induction.''' Two 0.20% Eiken agar tryptone plates supplemented with Amp were used in this assay. Swarms were allowed to grow for 6 hrs. (A) Non-induced plate. (B) Plate induced with IPTG. Colony Assignments: 1, RP437 positive control; 2, RP3087 negative control; 3, RP3087 containing pDB28; 4, RP3087 containing Construct C; 5, RP3087 containing Construct D; 6, RP3087 containing Construct E. Swarms were traced using the Adobe Photoshop computer program to easily identify their sizes. Construct C swarms only on the induced plate, whereas Constructs D and E swarm on both the induced and non-induced plates. </ins>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|<del class="diffchange diffchange-inline">Induced Motility</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Constructs C, D, and E, which contained Construct B and constitutively expressed lacI, were built to determine the effect of increased repressor concentration on the output device. Based on the results of the assay, Construct C was the only device shown to have an effective immotile state. The pLacIQ promoter used in this construct proved to be the only promoter strong enough to produce LacI at a concentration sufficient to fully repress cell motility in the non-induced state. It was also concluded that the pKat promoter used in Construct E was strong enough to produce a less effective “off” state. This was proven by the fact that colony on the non-induced plate was noticeable smaller than the colony on the induced plate. The pCat promoter used in Construct D was not strong enough to even slightly repress the swarming phenotype in the non-induced state. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''</ins></div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82482&oldid=prevLuw134 at 20:00, 27 October 20062006-10-27T20:00:03Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:00, 27 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|Induced Motility]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|Induced Motility]]</div></td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134http://www.openwetware.org/index.php?title=IGEM:PennState/2006/Progress_motility&diff=82477&oldid=prevLuw134 at 19:52, 27 October 20062006-10-27T19:52:14Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:52, 27 October 2006</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|Induced Motility]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:InducedMotilitywithIPTGPSUIGEM.jpg|left|Induced Motility]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:PSUigemconceptcartoon.jpg|left|PSU IGEM Cartoon]]</del></div></td><td colspan="2"> </td></tr>
<!-- diff generator: internal 2013-05-23 18:32:40 -->
</table>Luw134