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[[Image:Psuigemlogo2007.jpg]]
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<p>The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT.  The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006  
<p>The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT.  The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006  
jamboree consisted of 37 teams from 12 countries whom presented their findings.</p>
jamboree consisted of 37 teams from 12 countries whom presented their findings.</p>
<p>The PSU 2007 iGEM team is currently developing projects radiation detection and ethanol production. More information can be found in the Project Section.</p>  
<p>
Increasing energy demands have brought about the need for a renewable, efficient energy source.  Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie.  Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests.  Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose.  The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.</p>  
<p>If you are interested in joining the team [[IGEM:PennState/Newmember|click here]].</p></font>
<p>If you are interested in joining the team [[IGEM:PennState/Newmember|click here]].</p></font>


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<html><blink>></blink></html>People</font>
<html><blink>></blink></html>People</font>


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Fulltime Undergraduates</font></h2>
<h2>Fulltime Undergraduates</h2>
*[[user:nrj5011|<font color="#CCFFFF">Noah Johnson</font>]]
*[[user:nrj5011|<font color="#66CC66">Noah Johnson</font>]]  
*[[user:ALeung|<font color="#CCFFFF">Audrey Leung</font>]]
*[[user:tachyon60|<font color="#CCFFFF">Galen Lynch</font>]]  
*[[user:tachyon60|<font color="#CCFFFF">Galen Lynch</font>]]  
*[[user:GTobin|<font color="#CCFFFF">Garret Tobin]]
*[[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[user:luw134|<font color="#CCFFFF">Lucien Weiss]]
*[[user:luw134|<font color="#CCFFFF">Lucien Weiss</font>]]
*[[user:ALeung|<font color="#CCFFFF">Audrey Leung]]
*[[user:samhita_banavar|<font color="#CCFFFF">Samhita Banavar</font>]]


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2>


<h2>Part time Undergraduates</h2>
*[[IGEM:PennState/2006/user:Cbuck|<font color="#CCFFFF">Chris Buckno]]
*[[IGEM:PennState/2006/user:Cbuck|<font color="#CCFFFF">Chris Buckno]]
*[[IGEM:PennState/2006/user:Jgrim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/2006/user:Jgrim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/2006/user:Jbig|<font color="#CCFFFF">John Bigus</font>]]  
*[[IGEM:PennState/2006/user:Jbig|<font color="#CCFFFF">John Bigus</font>]]  
*[[IGEM:PennState/2006/user:Jdun|<font color="#CCFFFF">Jen Dunning</font>]]
*[[IGEM:PennState/2006/user:Jdun|<font color="#CCFFFF">Jen Dunning</font>]]
*[[IGEM:PennState/2007/user:GKho|<font color="#CCFFFF">George Khoury</font>]]


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Graduate Students</font></h2>
<h2>Graduate Students</h2>
*[[IGEM:PennState/2006/user:Megan_Marshall|<font color="#CCFFFF">Megan Marshall</font>]]
*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Megan Marshall</font>]]
*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Deepti Tanjore</font>]]
*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Deepti Tanjore</font>]]
*[[IGEM:PennState/2006/user:SWal|<font color="#CCFFFF">Steve Walker</font>]]
*[[IGEM:PennState/2006/user:SWal|<font color="#CCFFFF">Steve Walker</font>]]


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Faculty</font></h2>
<h2>Faculty</h2>
 
*[[IGEM:PennState/2006/user:Pcir|<font color="#CCFFFF">Pat Cirino</font>]]   
*[[IGEM:PennState/2006/user:Pcir|<font color="#CCFFFF">Pat Cirino</font>]]   
*[[IGEM:PennState/2006/user:Dfarb|<font color="#CCFFFF">Darryl Farber</font>]]   
*[[IGEM:PennState/2006/user:Dfarb|<font color="#CCFFFF">Darryl Farber</font>]]   
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*[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]]
*[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]]


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Photos</font></h2>


<h2>Photos</h2>
*[[IGEM:PennState/2007/Lab-photos|<font color="#CCFFFF">Lab Photos</font>]]   
*[[IGEM:PennState/2007/Lab-photos|<font color="#CCFFFF">Lab Photos</font>]]   
*[[IGEM:PennState/2007/Team-member-photos|<font color="#CCFFFF">Team Member Photos</font>]]   
*[[IGEM:PennState/2007/Team-member-photos|<font color="#CCFFFF">Team Member Photos</font>]]   
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*[[IGEM:PennState/2007/Graphics|<font color="#CCFFFF">Graphics</font>]]
*[[IGEM:PennState/2007/Graphics|<font color="#CCFFFF">Graphics</font>]]


<font color="#FFFFFF">
<h2><font color="#FFFFFF">Lab Notebooks</font></h2>
<h2>Lab Notebooks</h2>
 
*[[IGEM:PennState/Labbook/CommonBook|<font color="#CCFFFF">General Lab Notebook</font>]]
*[[IGEM:PennState/Labbook/ChrisBuckno|<font color="#CCFFFF">Chris Buckno</font>]]
*[[IGEM:PennState/Labbook/JoelQ.Grim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/Labbook/JoelQ.Grim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/Labbook/NoahJohnson|<font color="#CCFFFF">Noah Johnson</font>]]
*[[IGEM:PennState/Labbook/NoahJohnson|<font color="#CCFFFF">Noah Johnson</font>]]
*[[IGEM:PennState/Labbook/AudreyLeung|<font color="#CCFFFF">Audrey Leung</font>]]
*[[IGEM:PennState/Labbook/GalenLynch|<font color="#CCFFFF">Galen Lynch</font>]]  
*[[IGEM:PennState/Labbook/GalenLynch|<font color="#CCFFFF">Galen Lynch</font>]]  
*[[IGEM:PennState/Labbook/GarrettTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[IGEM:PennState/Labbook/GarrettTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[IGEM:PennState/Labbook/LucienWeiss|<font color="#CCFFFF">Lucien Weiss</font>]]
*[[IGEM:PennState/Labbook/LucienWeiss|<font color="#CCFFFF">Lucien Weiss</font>]]
*[[IGEM:PennState/Labbook/SamhitaBanavar|<font color="#CCFFFF">Samhita Banavar</font>]]
<h2><font color="#FFFFFF">Fun Stuff</font></h2>
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<h2>Fun Stuff</h2>


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<font color="#646B86" size="6"><html><blink>></blink></html>Project</font>
<font color="#646B86" size="6"><html><blink>></blink></html>Project</font>


<font color="#66CC66">
<h2>In The News</h2>
<h2>In The News</h2>


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*[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="#000000">MRSEC Publications</font>]
*[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="#000000">MRSEC Publications</font>]


<h2 style="color: #D16349">'''Tasks'''</h2>
<h2>Tasks</h2>


*[[IGEM:PennState/News/WebsiteTasks|<font color="#000000">Website Tasks</font>]]
*[[IGEM:PennState/News/WebsiteTasks|<font color="#000000">Website Tasks</font>]]
*[[IGEM:PennState/2007/News/MeetingNotes|<font color="#000000">Group Meeting Minutes</font>]]
*[[IGEM:PennState/2007/News/MeetingNotes|<font color="#000000">Group Meeting Minutes</font>]]


<h2 style="color: #D16349">'''Protocols'''</h2>
<h2>Protocols</h2>
 
*[[IGEM:PennState/2006/Growthmedia|<font color="#000000">Growth Media</font>]]
*[[IGEM:PennState/2006/Cloning|<font color="#000000">Cloning</font>]]  
*[[IGEM:PennState/2006/Cloning|<font color="#000000">Cloning</font>]]  
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000000">Preparing chemically competent cells</font>]]   
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000000">Preparing chemically competent cells</font>]]   
**[[IGEM:PennState/2006/GrowingParts|<font color="#000000">Growing Parts</font>]]  
**[[IGEM:PennState/2006/GrowingParts|<font color="#000000">Growing Parts</font>]]  
**[[IGEM:PennState/2007/Dnaprep|<font color="#000000">Telt Method DNA Prep</font>]]
**[[IGEM:PennState/2006/Restriction|<font color="#000000">Restriction</font>]]  
**[[IGEM:PennState/2006/Restriction|<font color="#000000">Restriction</font>]]  
**[[IGEM:PennState/2006/Ligation|<font color="#000000">Ligation</font>]]  
**[[IGEM:PennState/2006/Ligation|<font color="#000000">Ligation</font>]]  
**[[IGEM:PennState/2006/Transformation|<font color="#000000">Transformation</font>]]
**[[IGEM:PennState/2006/Transformation|<font color="#000000">Transformation</font>]]
*[[IGEM:PennState/2006/Lambda Techniques|<font color="#000000">Lambda Techniques</font>]]
*[[IGEM:PennState/2006/dnagels|<font color="#000000">Agarose Gel</font>]]
**[[IGEM:PennState/2006/sybrgreen|<font color="#000000">Working with Sybr Green I</font>]]
*[http://openwetware.org/wiki/Silver:_PCR <font color="#000000">PCR</font>]
**[[IGEM:PennState/2007/FreezeandSqueeze|<font color="#000000">PCR Purification Freeze and Squeeze</font>]]
*[[IGEM:PennState/2006/Plate-Reading|<font color="#000000">Plate-reading</font>]]
*[[IGEM:PennState/2006/Plate-Reading|<font color="#000000">Plate-reading</font>]]
*[[IGEM:PennState/2006/StrainConstruction|<font color="#000000">Strain Construction</font>]]   
*[[IGEM:PennState/2006/StrainConstruction|<font color="#000000">Strain Construction</font>]]   
*[[IGEM:PennState/2006/Eikenplates|<font color="#000000">Eiken Agar Plates</font>]]
*[[IGEM:PennState/2006/PSUinventory|<font color="#000000">Inventory</font>]]
*[[IGEM:PennState/2006/PSUinventory|<font color="#000000">Inventory</font>]]
*[[IGEM:PennState/2006/dnagels|<font color="#000000">DNA Gel</font>]]
*[[IGEM:PennState/2006/Growthmedia|<font color="#000000">Growth Media</font>]]
*[[IGEM:PennState/2006/LBPlates|<font color="#000000">LB Plates</font>]]
*[[IGEM:PennState/2006/Eikenplates|<font color="#000000">Eiken Agar Plates</font>]]
<h2 style="color:#D16349">'''Progress'''</h2>


<h2>Progress</h2>
*[[IGEM:PennState/2007/Progress_Project_Development|<font color="#000000">Project Development Overview</font>]]
*[[IGEM:PennState/2007/Progress_Project_Development|<font color="#000000">Project Development Overview</font>]]
*[[IGEM:PennState/2007/Progress_Cloning|<font color="#000000">Cloning</font>]]
*[[IGEM:PennState/2007/Progress_Cloning|<font color="#000000">Cloning</font>]]
*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000000">Submitted Registry Parts</font>]]
*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000000">Submitted Registry Parts</font>]]
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000000">Microcentrifuge Tube Lists</font>]]
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000000">Microcentrifuge Tube Lists</font>]]
*[[IGEM:PennState/2006/Progress_microchannels|<font color="#000000">Microchannels results</font>]]
*[[IGEM:PennState/2006/Progress_motility|<font color="#000000">Motility results</font>]]
*[[IGEM:PennState/2006/Progress_motility|<font color="#000000">Motility results</font>]]
*[[IGEM:PennState/2007/Storage|<font color="#000000">Glycerol Stock Inventory</font>]]


<h2 style="color: #D16349">'''Background'''</h2>
<h2>Background</h2>
*[[IGEM:PennState/history|<font color="#000000">History of Penn State iGEM</font>]]
*[[IGEM:PennState/history|<font color="#000000">History of Penn State iGEM</font>]]


<h2 style="color: #D16349">'''Links'''</h2>
<h2>Links</h2>
*[[iGEM|iGEM on OpenWetWare]]
*[http://parts.mit.edu/registry Parts registry]
*[http://parts.mit.edu/registry Parts registry]
*[http://parts.mit.edu/igem iGEM wiki]
*[http://parts.mit.edu/igem iGEM wiki]
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*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]
*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]
 
*[http://tanager.biotec.psu.edu/ Oligo orders]
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 Lambda Phage Genome]
*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html Ethanol Precipitation]
*[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi]
*[http://www.sciencegateway.org/tools/bacteria.htm OD Calculator]
|}
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Latest revision as of 12:01, 3 January 2008

The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006 jamboree consisted of 37 teams from 12 countries whom presented their findings.

Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.

If you are interested in joining the team click here.

<html><blink>></blink></html>People

Fulltime Undergraduates

Affiliated Undergraduates

Graduate Students

Faculty

Photos

Lab Notebooks


Fun Stuff


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<html><blink>></blink></html>Project

In The News

Tasks

Protocols

Progress

Background

Links

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