Nrj5011 at 16:50, 5 July 2007

2007-07-05T16:50:16Z

←Older revision		Revision as of 16:50, 5 July 2007
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-	'''Freeze 'N Squeeze DNA Gel Extraction'''	+	===<font color="red">'''Freeze 'N Squeeze DNA Gel Extraction'''</font>===

	1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1.		1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1.

Nrj5011: New page: '''Freeze 'N Squeeze DNA Gel Extraction''' 1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1....

2007-07-05T16:47:27Z

New page: '''Freeze 'N Squeeze DNA Gel Extraction''' 1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1....

New page

'''Freeze 'N Squeeze DNA Gel Extraction'''

1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1.

2. Using a clean razor blade, carefully excise the band of interest. Trim excess agarose from all six sides of the DNA band to maximize recovery and purity.

3. Chop the trimmed gel slice and place the pieces into the filter cup of the Quantum Prep Freeze 'N Squeeze DNA Gel Extraction Spin Column. Place the filter cup into the dolphin tube.

If the volume of your trimmed gel slice is too great to fit into one filter cup, then usetwo or more and pool the recovered samples ant the end of the protocol.

4. Place the Quantum Prep Freeze 'N Squeeze DNA Gel Extraction Spin Column (filter cup nested with dolphin tube) in a -20C freezer for five minutes.

5. Spin the sample at 13,000 x g for 3 minutes at room tempature.

6. Collect the purified DNA from the collection tube; the agarose debris will be retained within the filter cup of the Quantum Prep Freeze 'N Squeeze DNA Gel Extraction Spin Column. The DNA is read to use for PCR, ligations, labeling or other enzymatic reactions. Ethanol precipitation is recommeded for applications requiring a more concentrated sample and will also have the effect of further purifying the sample.

IGEM:PennState/2007/FreezeandSqueeze - Revision history

Nrj5011 at 16:50, 5 July 2007

Nrj5011: New page: '''Freeze 'N Squeeze DNA Gel Extraction''' 1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1....