IGEM:PennState/2007/Labbook/GarrettTobin/2007-7-26: Difference between revisions
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(New page: '''July 26th''' '''Plan for xylR''' *Make 6 different promoter+RBS+xylR constructs with different levels of expression **2 different RBS - B0033 (stregnth .01) - B0034 (streght 10 **3 diff...) |
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**Both into dh5a | **Both into dh5a | ||
**B0033 dna from regrestry plates is in my box- labled as exactly that | **B0033 dna from regrestry plates is in my box- labled as exactly that | ||
*Made sure there was a miniprep for other RBS-B0034 | *Made sure there was a miniprep for other RBS-B0034\ | ||
***12:30-5:30 | |||
Tomorrow | Tomorrow | ||
Latest revision as of 14:10, 31 July 2007
July 26th Plan for xylR
- Make 6 different promoter+RBS+xylR constructs with different levels of expression
- 2 different RBS - B0033 (stregnth .01) - B0034 (streght 10
- 3 different Promoters - 1, 200, 2000
Today:
- Transform xylR orf from miniprep
- Transfrom B0033
- Both into dh5a
- B0033 dna from regrestry plates is in my box- labled as exactly that
- Made sure there was a miniprep for other RBS-B0034\
- 12:30-5:30
Tomorrow
- Innoculate
- Prepare Transformation/Find Minipreps of 3 promoters
Saturday
- Miniprep, Restrict, Purify, Ligate, Transform
- Innoculate promoters if needed
Sunday
- Innoculate
