IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-2: Difference between revisions
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Primers | Primers | ||
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TOTAL: | TOTAL: | ||
Front | Front | ||
Gaattcgcggccgcttctagag AAACGCCGTT CTTGATTTTG | Gaattcgcggccgcttctagag AAACGCCGTT CTTGATTTTG | ||
| Line 52: | Line 55: | ||
Reverse complement | Reverse complement | ||
Ctgcagcggccgctactagta CTGATGTGACGCCGACAATT | Ctgcagcggccgctactagta CTGATGTGACGCCGACAATT | ||
---- | |||
Middle for sequencing: | Middle for sequencing: | ||
Forward | Forward | ||
GCTACAAACG CTACCACCGC | GCTACAAACG CTACCACCGC | ||
Revision as of 13:35, 2 August 2007
August 2nd
- Miniprep B0034 (2)
- Digest those with 2 xylR minipreps from yesterday
- Ran Gel
- Designed Primers for xylR Total and middle for sequencing
xylR total 3732929..3734180
Extended for primers 3732910…3734210
AAACGCCGTT CTTGATTTTG ATAAAAATTT TCTCAAAGCC GGTTACGTAT TACCGGTTTT GAGTTTTTGC ATGATTCAGC AGGAAAAGAA CCatgTTTAC TAAACGTCAC CGCATCACAT TACTGTTCAA TGCCAATAAA GCCTATGACC GGCAGGTAGT AGAAGGCGTA GGGGAATATT TACAGGCGTC ACAATCGGAA TGGGATATTT TCATTGAAGA AGATTTCCGC GCCCGCATTG ATAAAATCAA GGACTGGTTA GGAGATGGCG TCATTGCCGA CTTCGACGAC AAACAGATCG AGCAAGCGCT GGCTGATGTC GACGTCCCCA TTGTTGGGGT TGGCGGCTCG TATCACCTTG CAGAAAGTTA CCCACCCGTT CATTACATTG CCACCGATAA CTATGCGCTG GTTGAAAGCG CATTTTTGCA TTTAAAAGAG AAAGGCGTTA ACCGCTTTGC TTTTTATGGT CTTCCGGAAT CAAGCGGCAA ACGTTGGGCC ACTGAGCGCG AATATGCATT TCGTCAGCTT GTCGCCGAAG AAAAGTATCG CGGAGTGGTT TATCAGGGGT TAGAAACCGC GCCAGAGAAC TGGCAACACG CGCAAAATCG GCTGGCAGAC TGGCTACAAA CGCTACCACC GCAAACCGGG ATTATTGCCG TTACTGACGC CCGAGCGCGG CATATTCTGC AAGTATGTGA ACATCTACAT ATTCCCGTAC CGGAAAAATT ATGCGTGATT GGCATCGATA ACGAAGAACT GACCCGCTAT CTGTCGCGTG TCGCCCTTTC TTCGGTCGCT CAGGGCGCGC GGCAAATGGG CTATCAGGCG GCAAAACTGT TGCATCGATT ATTAGATAAA GAAGAAATGC CGCTACAGCG AATTTTGGTC CCACCAGTTC GCGTCATTGA ACGGCGCTCA ACAGATTATC GCTCGCTGAC CGATCCCGCC GTTATTCAGG CCATGCATTA CATTCGTAAT CACGCCTGTA AAGGGATTAA AGTGGATCAG GTACTGGATG CGGTCGGGAT CTCGCGCTCC AATCTTGAGA AGCGTTTTAA AGAAGAGGTG GGTGAAACCA TCCATGCCAT GATTCATGCC GAGAAGCTGG AGAAAGCGCG CAGTCTGCTG ATTTCAACCA CCTTGTCGAT CAATGAGATA TCGCAAATGT GCGGTTATCC ATCGCTGCAA TATTTCTACT CTGTTTTTAA AAAAGCATAT GACACGACGC CAAAAGAGTA TCGCGATGTA AATAGCGAGG TCATGTTGTA GCGATGATGA GAATTGTCGG CGTCACATCA G
Primers
TOTAL:
Front Gaattcgcggccgcttctagag AAACGCCGTT CTTGATTTTG
End AATTGTCGG CGTCACATCAG tactagtagcggccgctgcag
Reverse complement Ctgcagcggccgctactagta CTGATGTGACGCCGACAATT
Middle for sequencing: Forward GCTACAAACG CTACCACCGC
Reverse Compliment GCGGTGGTAGCGTTTGTAGC
