IGEM:PennState/2007/Microcentrifuge tube lists/GT: Difference between revisions

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#Tube 0001
#Tube 0001-Thrown Away
#*J23038; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
#*J23038; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
#*RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
#*RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J23038 Registry Part Page]]
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J23038 Registry Part Page]]
#Tube 0002
#Tube 0002-Thrown Away
#*J31004; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
#*J31004; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
#*Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
#*Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J31004 Registry Part Page]]
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J31004 Registry Part Page]]
#Tube 0003
#*B0033; From Regestry Plates 7/26
#* RBS (.01 stregnth)
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_B0033 Registry Part Page]]
#Tube 0005
#*xylR ORF miniprep 7/31 (1)
#*Part:BBa_I741005
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_I741005 Registry Part Page]]
#Tube 0006
#*xylR ORF miniprep 7/31 (3 different colony)
#*Part:BBa_I741005
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_I741005 Registry Part Page]]
#Tube 0007
#*xylR ORF extraction 7/31 (1)
#*Part:BBa_I741005, cut X + P
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_I741005 Registry Part Page]]
#Tube 0008
#*xylR ORF extraction 7/31 (3)
#*Part:BBa_I741005, cut X + P
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_I741005 Registry Part Page]]
#Tube 0009
#*xylR ORF extraction 8/2
#* RBS (.01 stregnth) cut S+P
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_B0033 Registry Part Page]]
#Tube 0010
#*xylR ORF extraction 8/2
#*Part:BBa_I741005, cut X + P
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_I741005 Registry Part Page]]
#Tube 0011
#*xylR ORF+B0034 ligation 8/3
#*3 vector 3 B0034
#Tube 0012
#*xylR ORF+B0034 ligation 8/3
#*8 vector .5 B0034
#Tube 0013
#*xylR ORF+B0034 ligation 8/3
#*9 vector 3 B0034
#Tube 0014
#*xylR ORF+B0034 ligation 8/3
#*4 vector .5 B0034
#Tube 0015
#*xylR ORF+B0034 ligation 8/3
#*12.5 vector 2.5 B0034

Latest revision as of 12:14, 6 August 2007

  1. Tube 0001-Thrown Away
  2. Tube 0002-Thrown Away
  3. Tube 0003
  4. Tube 0005
  5. Tube 0006
  6. Tube 0007
  7. Tube 0008
  8. Tube 0009
  9. Tube 0010
  10. Tube 0011
    • xylR ORF+B0034 ligation 8/3
    • 3 vector 3 B0034
  11. Tube 0012
    • xylR ORF+B0034 ligation 8/3
    • 8 vector .5 B0034
  12. Tube 0013
    • xylR ORF+B0034 ligation 8/3
    • 9 vector 3 B0034
  13. Tube 0014
    • xylR ORF+B0034 ligation 8/3
    • 4 vector .5 B0034
  14. Tube 0015
    • xylR ORF+B0034 ligation 8/3
    • 12.5 vector 2.5 B0034
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