IGEM:PennState/2007/Microcentrifuge tube lists/GT

1. Tube 0001-Thrown Away
   • J23038; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
   • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
   • [Registry Part Page]
2. Tube 0002-Thrown Away
   • J31004; Extracted 5-24-07 from gel http://openwetware.org/wiki/IGEM:PennState/Labbook/LucienWeiss#May_24.2C_2007:_Gel_Results:_J32038_.26_J31004_Digests]; on plasmid PSB1A2; In -4C Box GT-1
   • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
   • [Registry Part Page]
3. Tube 0003
   • B0033; From Regestry Plates 7/26
   • RBS (.01 stregnth)
   • [Registry Part Page]
4. Tube 0005
   • xylR ORF miniprep 7/31 (1)
   • Part:BBa_I741005
   • [Registry Part Page]
5. Tube 0006
   • xylR ORF miniprep 7/31 (3 different colony)
   • Part:BBa_I741005
   • [Registry Part Page]
6. Tube 0007
   • xylR ORF extraction 7/31 (1)
   • Part:BBa_I741005, cut X + P
   • [Registry Part Page]
7. Tube 0008
   • xylR ORF extraction 7/31 (3)
   • Part:BBa_I741005, cut X + P
   • [Registry Part Page]
8. Tube 0009
   • xylR ORF extraction 8/2
   • RBS (.01 stregnth) cut S+P
   • [Registry Part Page]
9. Tube 0010
   • xylR ORF extraction 8/2
   • Part:BBa_I741005, cut X + P
   • [Registry Part Page]
10. Tube 0011
    • xylR ORF+B0034 ligation 8/3
    • 3 vector 3 B0034
11. Tube 0012
    • xylR ORF+B0034 ligation 8/3
    • 8 vector .5 B0034
12. Tube 0013
    • xylR ORF+B0034 ligation 8/3
    • 9 vector 3 B0034
13. Tube 0014
    • xylR ORF+B0034 ligation 8/3
    • 4 vector .5 B0034
14. Tube 0015
    • xylR ORF+B0034 ligation 8/3
    • 12.5 vector 2.5 B0034