IGEM:PennState/2007/Microcentrifuge tube lists/LW: Difference between revisions

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#*RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
#*RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J23038 Registry Part Page]]
#*[[http://parts.mit.edu/registry/index.php/Part:BBa_J23038 Registry Part Page]]
#*
#Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
#*A newer version of this is available see LW0016
#*
#Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
#*Currently used in PCR: experience successful with Pfx and Pfu
#*
#50mM MgSO4; filter steralized; prepared 6-??-07
#*Experience: successful for PCR; use MgSO4 that is in Pfx package when doing PCR
#*note Pfu buffer already has MgSO4 in it.
#*
#*
#
#

Revision as of 06:24, 10 July 2007

  1. J23038; Transformed 5-23-07 from registry plate 2006-1 well 9G; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  2. J31004; Transformed 5-23-07 from registry plate 2006-1 well 4E; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  3. J31004 A; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  4. J31004 B; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  5. J31004 C; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  6. J23038 A; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  7. J23038 B; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  8. J23038 C; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  9. Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
    • A newer version of this is available see LW0016
  10. Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
    • Currently used in PCR: experience successful with Pfx and Pfu
  11. 50mM MgSO4; filter steralized; prepared 6-??-07
    • Experience: successful for PCR; use MgSO4 that is in Pfx package when doing PCR
    • note Pfu buffer already has MgSO4 in it.
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