IGEM:PennState/2007/Microcentrifuge tube lists/LW

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  1. J23038; Transformed 5-23-07 from registry plate 2006-1 well 9G; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  2. J31004; Transformed 5-23-07 from registry plate 2006-1 well 4E; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  3. J31004 A; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  4. J31004 B; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  5. J31004 C; MP 5-24-07 from Transformed DNA LW0002 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  6. J23038 A; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  7. J23038 B; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  8. J23038 C; MP 5-24-07 from Transformed DNA LW0001 LW-1; on plasmid PSB1A2; In -20C Box LW1
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  9. J23038A; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  10. J23038B; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  11. J23038C; EX(gel extracted) EcoRI+SPEI 5-25-07 from LW006 LW-1;
    • RA2 promoter from 1022 library with a ribosome binding site. A composite part of J23104 and B0034
    • [Registry Part Page]
  12. J31004A; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  13. J31004B; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  14. J31004C; EX(gel Extracted) ECORI+XBAI 5-25-07 from LW0003 LW-1;
    • Codes the reverse of the CmR gene. If the region has a promoter and an RBS in front and is flipped, the cell will express Chloramphenicol resistance.
    • [Registry Part Page]
  15. Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
    • A newer version of this is available see LW0016
  16. Chromosomal DNAPrep; Telt Prep 6-??-07 from strain W3110; in -20 Box LW1
    • Currently used in PCR: experience successful with Pfx and Pfu
  17. 50mM MgSO4; filter steralized; prepared 6-??-07
    • Experience: successful for PCR; use MgSO4 that is in Pfx package when doing PCR
    • note Pfu buffer already has MgSO4 in it.
  18. Sender Construct; MP 7-5-07 from glycerol stock;
    • attempted use Digest and religate into pSB1AK3: attempt failed
    • Similar construct with better ribosome binding site on registry plate 2-2007
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