IGEM:PennState/2007/Progress Cloning: Difference between revisions
(New page: Primers: <h2>Promoter Region Primers</h2> F-GGAATTCGCGGCCGCTTCTAGAG *TmC R-CTGCAGCGGCCGCTACTAGTA *TmC <h3>Double Promoter</h3> Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATA...) |
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Primers: | Primers: | ||
<h2>Promoter Region Primers</h2> | <h2>Promoter Region Primers</h2> | ||
<h3>Double Promoter</h3> | <h3>Double Promoter</h3> | ||
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*TmC 57 | *TmC 57 | ||
< | <h2>XylR</h2> | ||
XylR with Promoter RBS and terminator: Right | |||
F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG | |||
*TmC 65 | |||
R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC | |||
*TmC 63 | |||
XylR with Promoter RBS and terminator: Left | |||
F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC | |||
*TmC 63 | |||
R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG | |||
*TmC 65 | |||
XylR ORF Right | |||
**Missing** | |||
XylR ORF Left | |||
**Missing** | |||
Latest revision as of 12:27, 12 June 2007
Primers:
Promoter Region Primers
Double Promoter
Full Promoter AtoF F-GGAATTCGCGGCCGCTTCTAGAGTATTGAACTCCATAATCAGGTAATG
- TmC 61
R-CTGCAGCGGCCGCTACTAGTATGGTGTAGGGCCTTCTGTAG
- TmC 62
Full Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGTGGTGTAGGGCCTTCTGTA
- TmC 61
R-CTGCAGCGGCCGCTACTAGTATATTGAACTCCATAATCAGGTAATG
- TmC 58
ORF Promoter AtoF
- MISSING**
ORF Promoter FtoA F-GGAATTCGCGGCCGCTTCTAGAGAACCAATTTTTAGCAACTAAACAG
- TmC 58
R-CTGCAGCGGCCGCTACTAGTAATGGATGATGTCGTAATATTGG
- TmC 59
Single Promoter
CRP XylR XylR Fis F- GGAATTCGCGGCCGCTTCTAGAGTCCCTTTCAGTTGCTCAAT
- TmC 58
R- CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG
- TmC 60
XYLR XYLR FIS F-GGAATTCGCGGCCGCTTCTAGAGTTACGTTTATCGCGGTGATT
- TmC 59
R-CTGCAGCGGCCGCTACTAGTAGGTGTAGGGCCTTCTGTAG
- TmC 60
CRP
F-GGAATTCGCGGCCGCTTCTAGAGATGGTGCTTGGCAAACC
- TmC 60
R-CTGCAGCGGCCGCTACTAGTATTAACGAGTGCCGTAAAC
- TmC 57
XylR
XylR with Promoter RBS and terminator: Right F-GGAATTCGCGGCCGCTTCTAGAGGCAACCAAACGCCGTTCTTG
- TmC 65
R-CTGCAGCGGCCGCTACTAGTAATTGCATTTCCTTGAGCCTTATCC
- TmC 63
XylR with Promoter RBS and terminator: Left F-GGAATTCGCGGCCGCTTCTAGAGATTGCATTTCCTTGAGCCTTATCC
- TmC 63
R-CTGCAGCGGCCGCTACTAGTAGCAACCAAACGCCGTTCTTG
- TmC 65
XylR ORF Right
- Missing**
XylR ORF Left
- Missing**
