IGEM:PennState/2008: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
mNo edit summary
Line 171: Line 171:
<h2>Protocols</h2>
<h2>Protocols</h2>
*[[IGEM:PennState/2006/Growthmedia|<font color="#000">Growth Media</font>]]
*[[IGEM:PennState/2006/Growthmedia|<font color="#000">Growth Media</font>]]
*[[IGEM:PennState/2006/Cloning|<font color="#000">Cloning</font>]]  
*[[IGEM:PennState/2006/Cloning|<font color="#000">Cloning</font>]]   
**[[IGEM:PennState/2006/GrowingParts|<font color="#000">Growing Parts</font>]]  
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000">Preparing chemically competent cells</font>]]
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000">Preparing chemically competent cells</font>]]
**[[IGEM:PennState/2008/PreparingElectrocompetentCells|<font color="#000">Preparing Electrocompetent cells</font>]] 
**[[IGEM:PennState/2008/PreparingElectrocompetentCells|<font color="#000">Preparing Electrocompetent cells</font>]]
**[[IGEM:PennState/2006/GrowingParts|<font color="#000">Growing Parts</font>]]  
**[[IGEM:PennState/2007/Dnaprep|<font color="#000">Telt Method DNA Prep</font>]]  
**[[IGEM:PennState/2007/Dnaprep|<font color="#000">Telt Method DNA Prep</font>]]  
**[[IGEM:PennState/2006/Restriction|<font color="#000">Restriction</font>]]  
**[[IGEM:PennState/2006/Restriction|<font color="#000">Restriction</font>]]  

Revision as of 19:51, 14 July 2008

<html> <head> <style type="text/css"> .teamm {

border-right: dashed 1px #fff;
width: 16em;

} </style> </head> </html>


What iGEM is All About

The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of an open registry of parts, or biobricks. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The [2007 Jamboree] consisted of 54 teams from 19 countries who presented their findings.

Penn State's 2007 iGEM project: Diauxie Elimination

Penn State won a Gold medal in the 2007 iGEM Jamboree! We're proud of our work during the year and our registry submissions.

Abstract:

Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.

Want to Join?

If you are interested in joining the team click here.




<html><blink>></blink></html>People

Fulltime Undergraduates

Garrett Tobin Lab Notebook
Trevor Wiley Lab Notebook
Nimrah Ahmed Lab Notebook
Daniel Seong Lab Notebook
Shariful Alam Lab Notebook
Berooke Alemayehu Lab Notebook

High School Student

Samhita Banavar Lab Notebook

Affiliated Undergraduates

George Khoury
Galen Lynch Lab Notebook

Advisory Assistance

Faculty


Fun Stuff


<html><a href="http://www2.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/IGEM:PennState&type=small&category=free&clusters=no&map=world" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/IGEM:PennState" /></a> <br> <br>

<!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=2623386; var sc_invisible=0; var sc_partition=25; var sc_security="dc27ec2c"; </script>

<a href="http://my.statcounter.com/project/standard/stats.php?project_id=2623386&guest=1" id="clustrMapsLink"><img src="http://c26.statcounter.com/counter.php?sc_project=2623386&java=0&security=dc27ec2c&invisible=0" alt="blog counter" border="0"" /></a> <br> <br> <SCRIPT LANGUAGE="JAVASCRIPT"><!-- hide this script tag's contents from old browsers

today = new Date();

BigDay = new Date("November 3, 2007") msPerDay = 24 * 60 * 60 * 1000 ; timeLeft = (BigDay.getTime() - today.getTime()); e_daysLeft = timeLeft / msPerDay; daysLeft = Math.floor(e_daysLeft); e_hrsLeft = (e_daysLeft - daysLeft)*24; hrsLeft = Math.floor(e_hrsLeft); minsLeft = Math.floor((e_hrsLeft - hrsLeft)*60); document.write(<font color=red>"There are only:<BR> <H4>" + daysLeft + " days " + hrsLeft +" hours and " + minsLeft + " minutes left </H4> Until the 2007 iGEM Jamboree! (Nov. 3rd, 2007)<P>"</font>);

// -- done hiding from old browsers --></SCRIPT>

</html>









<html><font face="Tahoma" color="#FFFFFF" size="6"><blink>></blink>Project</font> </html>

In The News

Tasks


Team Utilities

Protocols

Progress

Background

Links