IGEM:PennState/2008

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<html><font face="Tahoma" color="#FFFFFF" size="6"><blink>></blink>Project</font>
 
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*[[IGEM:PennState/Labbook/CommonBook|<font color="#CCFFFF">General Lab Notebook</font>]]
 
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<h2>In The News</h2>
 
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*[[IGEM:PennState/Publications/Synthetic Sports: A Bacterial Relay Race|<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]]
 
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*[[IGEM:PennState/Publications/Posters|<font color="#000">PSU iGEM Posters</font>]]
 
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*[https://www.engr.psu.edu/NewsEvents/EPS/v22n2_2006spring/igem.htm<font color="#000">Ready. Set. Grow!?</font>]
 
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*[http://www.ainewsletter.com/newsletters/aix_0512.htm#biobricks_conjugate <font color="#000">AI Newsletter</font>]
 
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*[http://publications.nigms.nih.gov/findings/mar07/findings_mar07.pdf <font color="#000">Government Report</font>]
 
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*[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="#000">MRSEC Publications</font>]
 
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<h2>Tasks</h2>
 
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*[[IGEM:PennState/News/WebsiteTasks|<font color="#000">Website Tasks</font>]]
 
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*[[IGEM:PennState/2007/News/MeetingNotes|<font color="#000">Group Meeting Minutes</font>]]
 
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<h2>Protocols</h2>
 
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*[[IGEM:PennState/2006/Growthmedia|<font color="#000">Growth Media</font>]]
 
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*[[IGEM:PennState/2006/Cloning|<font color="#000">Cloning</font>]]
 
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**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000">Preparing chemically competent cells</font>]]
 
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**[[IGEM:PennState/2008/PreparingElectrocompetentCells|<font color="#000">Preparing Electrocompetent cells</font>]] 
 
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**[[IGEM:PennState/2006/GrowingParts|<font color="#000">Growing Parts</font>]]
 
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**[[IGEM:PennState/2007/Dnaprep|<font color="#000">Telt Method DNA Prep</font>]]
 
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**[[IGEM:PennState/2006/Restriction|<font color="#000">Restriction</font>]]
 
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**[[IGEM:PennState/2006/Ligation|<font color="#000">Ligation</font>]]
 
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**[[IGEM:PennState/2006/Transformation|<font color="#000">Transformation</font>]]
 
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*[[IGEM:PennState/2006/dnagels|<font color="#000">Agarose Gel</font>]]
 
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**[[IGEM:PennState/2008/TAEBuffer|<font color="#000">TAE Buffer</font>]]
 
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**[[IGEM:PennState/2006/sybrgreen|<font color="#000">Working with Sybr Green I</font>]]
 
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*[http://openwetware.org/wiki/Silver:_PCR <font color="#000">PCR</font>]
 
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**[[IGEM:PennState/2007/FreezeandSqueeze|<font color="#000">PCR Purification Freeze and Squeeze</font>]]
 
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*[[IGEM:PennState/2006/Plate-Reading|<font color="#000">Plate-reading</font>]]
 
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*[[IGEM:PennState/2006/StrainConstruction|<font color="#000">Strain Construction</font>]] 
 
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*[[IGEM:PennState/2006/Eikenplates|<font color="#000">Eiken Agar Plates</font>]]
 
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*[[IGEM:PennState/2006/PSUinventory|<font color="#000">Inventory</font>]]
 
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<h2>Progress</h2>
 
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*[[IGEM:PennState/2007/Progress_Project_Development|<font color="#000">Project Development Overview</font>]]
 
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*[[IGEM:PennState/2007/Progress_Cloning|<font color="#000">Cloning</font>]]
 
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*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000">Submitted Registry Parts</font>]]
 
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*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000">Microcentrifuge Tube Lists</font>]]
 
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*[[IGEM:PennState/2006/Progress_motility|<font color="#000">Motility results</font>]]
 
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*[[IGEM:PennState/2007/Storage|<font color="#000">Glycerol Stock Inventory</font>]]
 
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<h2>Background</h2>
 
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*[[IGEM:PennState/history|<font color="#000">History of Penn State iGEM</font>]]
 
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<h2>Links</h2>
 
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*[http://parts.mit.edu/registry  <font color="#000">Parts registry</font>]
 
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*[http://parts.mit.edu/igem iGEM  <font color="#000">wiki</font>]
 
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*[[iGEM:PennState/2006 <font color="#000">Penn State iGEM 2006 on OpenWetWare</font>]]
 
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*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State <font color="#000"> iGEM 2005</font>]
 
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*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 <font color="#000">Penn State iGEM 2006</font>]
 
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*[http://tanager.biotec.psu.edu/|<font color="#000"> Oligo orders</font>]
 
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 | <font color="#000">Lambda Phage Genome</font>]
 
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*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html <font color="#000"> Ethanol Precipitation</font>]
 
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*[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi]
 
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*[http://www.sciencegateway.org/tools/bacteria.htm <font color="#000"> OD Calculator</font>]
 
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<h2><font color="#FFFFFF">Fulltime Undergraduates</font></h2>
<h2><font color="#FFFFFF">Fulltime Undergraduates</font></h2>
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|style="border-right: dashed 1px #fff"| [[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
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|class="teamm"| [[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
|[[IGEM:PennState/Labbook/GarrettTobin08|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/GarrettTobin08|<font color="#CCFFFF">Lab Notebook</font>]]
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:Trevor Wiley|<font color="#CCFFFF">Trevor Wiley</font>]]
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|class="teamm"|[[user:Trevor Wiley|<font color="#CCFFFF">Trevor Wiley</font>]]
|[[IGEM:PennState/Labbook/Trevor Wiley|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/Trevor Wiley|<font color="#CCFFFF">Lab Notebook</font>]]
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:Nimrah Ahmed|<font color="#CCFFFF">Nimrah Ahmed</font>]]
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|class="teamm"|[[user:Nimrah Ahmed|<font color="#CCFFFF">Nimrah Ahmed</font>]]
|[[IGEM:PennState/Labbook/Nimrah Ahmed|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/Nimrah Ahmed|<font color="#CCFFFF">Lab Notebook</font>]]
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:yourusernamehere|<font color="#CCFFFF">Daniel Seong</font>]]
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|class="teamm"|[[user:yourusernamehere|<font color="#CCFFFF">Daniel Seong</font>]]
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:Shariful Alam|<font color="#CCFFFF">Shariful Alam</font>]]
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|class="teamm"|[[user:Shariful Alam|<font color="#CCFFFF">Shariful Alam</font>]]
|[[IGEM:PennState/Labbook/Shariful Alam|<font color="#CCFFFF">Lab Notebook</font>]]  
|[[IGEM:PennState/Labbook/Shariful Alam|<font color="#CCFFFF">Lab Notebook</font>]]  
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:samhita_banavar|<font color="#CCFFFF">Samhita Banavar</font>]]
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|class="teamm"|[[user:yourusernamehere|<font color="#CCFFFF">Berooke Alemayehu</font>]]
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|[[IGEM:PennState/Labbook/SamhitaBanavar|<font color="#CCFFFF">Lab Notebook</font>]]
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|-
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|style="border-right: dashed 1px #fff"|[[user:yourusernamehere|<font color="#CCFFFF">Brooke Alemayehu</font>]]
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|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
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<h2><font color="#FFFFFF">High School Student</font></h2>
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{|valign="top" style="background:none; border: 0; width: 500px; text-indent: 3em"
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|class="teamm"| [[user:samhita_banavar|<font color="#CCFFFF">Samhita Banavar</font>]]
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|[[IGEM:PennState/Labbook/GarrettTobin08|<font color="#CCFFFF">Lab Notebook</font>]]
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<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2>
<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2>
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|[[IGEM:PennState/2007/user:GKho|<font color="#CCFFFF">George Khoury</font>]] ||
|[[IGEM:PennState/2007/user:GKho|<font color="#CCFFFF">George Khoury</font>]] ||
|-
|-
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|style="border-right: dashed 1px #fff"|[[user:Galen Lynch|<font color="#CCFFFF">Galen Lynch</font>]]
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|class="teamm"|[[user:Galen Lynch|<font color="#CCFFFF">Galen Lynch</font>]]
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]]
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<html><font face="Tahoma" color="#FFFFFF" size="6"><blink>></blink>Project</font>
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</html>
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*[http://2008.igem.org/Team:PennState <font color="#CCFFFF">2008 Penn State iGEM Project Site</font>]
 +
*[http://2008.igem.org/Team:PennState/Notebook <font color="#CCFFFF">2008 Lab Notebook</font>]
 +
 +
<h2>In The News</h2>
 +
 +
*[[IGEM:PennState/Publications/Synthetic Sports: A Bacterial Relay Race|<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]]
 +
*[[IGEM:PennState/Publications/Posters <font color="#000">PSU iGEM Posters</font>]]
 +
*[https://www.engr.psu.edu/NewsEvents/EPS/v22n2_2006spring/igem.htm<font color="#000">Ready. Set. Grow!?</font>]
 +
*[http://www.ainewsletter.com/newsletters/aix_0512.htm#biobricks_conjugate <font color="#000">AI Newsletter</font>]
 +
*[http://publications.nigms.nih.gov/findings/mar07/findings_mar07.pdf <font color="#000">Government Report</font>]
 +
*[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="#000">MRSEC Publications</font>]
 +
 +
<h2>Tasks</h2>
 +
*[[IGEM:PennState/News/WebsiteTasks|<font color="#000">Website Tasks</font>]]
 +
*[[IGEM:PennState/2007/News/MeetingNotes|<font color="#000">Group Meeting Minutes</font>]]
 +
 +
 +
<h2>Team Utilities</h2>
 +
*[http://tools.neb.com/NEBcutter2/index.php <font color="#000">NEB Cutter</font>]
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*[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp <font color="#000">Double Digest Finder</font>]
 +
*[http://tanager.biotec.psu.edu/|<font color="#000">Oligo orders</font>]
 +
*[http://www.geneart.com/|<font color="#000">Plasmid Orders (Geneart)</font>]
 +
*[http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix|<font color="#000">BioBrick Prefix & Suffix</font>]
 +
*[http://www.sciencegateway.org/tools/bacteria.htm <font color="#000">OD Calculator</font>]
 +
 +
<h2>Protocols</h2>
 +
*[[IGEM:PennState/2006/Growthmedia|<font color="#000">Growth Media</font>]]
 +
*[[IGEM:PennState/2006/Cloning|<font color="#000">Cloning</font>]]   
 +
**[[IGEM:PennState/2006/GrowingParts|<font color="#000">Growing Parts (Innoculation)</font>]]
 +
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000">Preparing chemically competent cells</font>]]
 +
**[[IGEM:PennState/2008/PreparingElectrocompetentCells|<font color="#000">Preparing Electrocompetent cells</font>]]
 +
**[[IGEM:PennState/2007/Dnaprep|<font color="#000">Telt Method DNA Prep</font>]]
 +
**[[IGEM:PennState/2006/Restriction|<font color="#000">Restriction</font>]]
 +
**[[IGEM:PennState/2006/Ligation|<font color="#000">Ligation</font>]]
 +
**[[IGEM:PennState/2006/Transformation|<font color="#000">Transformation</font>]]
 +
*[[IGEM:PennState/2006/dnagels|<font color="#000">Agarose Gel</font>]]
 +
**[[IGEM:PennState/2008/TAEBuffer|<font color="#000">TAE Buffer</font>]]
 +
**[[IGEM:PennState/2006/sybrgreen|<font color="#000">Working with Sybr Green I</font>]]
 +
*[http://openwetware.org/wiki/Silver:_PCR <font color="#000">PCR</font>]
 +
**[[IGEM:PennState/2007/FreezeandSqueeze|<font color="#000">PCR Purification Freeze and Squeeze</font>]]
 +
*[[IGEM:PennState/2006/Plate-Reading|<font color="#000">Plate-reading</font>]]
 +
*[[IGEM:PennState/2006/StrainConstruction|<font color="#000">Strain Construction</font>]] 
 +
*[[IGEM:PennState/2006/Eikenplates|<font color="#000">Eiken Agar Plates</font>]]
 +
*[[IGEM:PennState/2006/PSUinventory|<font color="#000">Inventory</font>]]
 +
 +
<h2>Progress</h2>
 +
*[[IGEM:PennState/2007/Progress_Project_Development|<font color="#000">Project Development Overview</font>]]
 +
*[[IGEM:PennState/2007/Progress_Cloning|<font color="#000">Cloning</font>]]
 +
*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000">Submitted Registry Parts</font>]]
 +
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000">Microcentrifuge Tube Lists</font>]]
 +
*[[IGEM:PennState/2006/Progress_motility|<font color="#000">Motility results</font>]]
 +
*[[IGEM:PennState/2007/Storage|<font color="#000">Glycerol Stock Inventory</font>]]
 +
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<h2>Background</h2>
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*[[IGEM:PennState/history|<font color="#000">History of Penn State iGEM</font>]]
 +
*[[iGEM:PennState/2006 | <font color="#000">Penn State iGEM 2006 on OpenWetWare</font>]]
 +
*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State <font color="#000"> iGEM 2005</font>]
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*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 <font color="#000">Penn State iGEM 2006</font>]
 +
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<h2>Links</h2>
 +
*[http://parts.mit.edu/registry  <font color="#000">Parts registry</font>]
 +
*[http://parts.mit.edu/igem iGEM  <font color="#000">wiki</font>]
 +
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 | <font color="#000">Lambda Phage Genome</font>]
 +
*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html <font color="#000"> Ethanol Precipitation</font>]
 +
*[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi]
 +
 +
 +
 +
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Current revision

Image:Psuigemlogo2008.jpg

Image:PSUiGEM2007OldMain.jpg


What iGEM is All About

The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of an open registry of parts, or biobricks. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The [2007 Jamboree] consisted of 54 teams from 19 countries who presented their findings.

Penn State's 2007 iGEM project: Diauxie Elimination

Penn State won a Gold medal in the 2007 iGEM Jamboree! We're proud of our work during the year and our registry submissions.

Abstract:

Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.

Want to Join?

If you are interested in joining the team click here.

</font>




>People

Fulltime Undergraduates

Garrett Tobin Lab Notebook
Trevor Wiley Lab Notebook
Nimrah Ahmed Lab Notebook
Daniel Seong Lab Notebook
Shariful Alam Lab Notebook
Berooke Alemayehu Lab Notebook

High School Student

Samhita Banavar Lab Notebook

Affiliated Undergraduates

George Khoury
Galen Lynch Lab Notebook

Advisory Assistance

Faculty


Fun Stuff




blog counter









>Project

In The News

Tasks


Team Utilities

Protocols

Progress

Background

Links







Personal tools