IGEM:PennState/Labbook/GalenLynch/2007-6-11: Difference between revisions
No edit summary |
No edit summary |
||
Line 3: | Line 3: | ||
<h2>XylR with promoter and rbs and terminator (right)</h2> | <h2>XylR with promoter and rbs and terminator (right)</h2> | ||
<html><pre>ATTTTGATAA AAATTTTCTC AAAGCCGGTT ACGTATTACC GGTTTTGAGT TTTTGCATGA<br>TTCAGCAGGA AAAGAACC<font color=green>a</font><font color=green>t</font> <font color=green>g</font>TTTACTAAA CGTCACCGCA TCACATTACT GTTCAATGCC<br>AATAAAGCCT ATGACCGGCA GGTAGTAGAA GGCGTAGGGG AATATTTACA GGCGTCACAA<br>TCGGAATGGG ATATTTTCAT TGAAGAAGAT TTCCGCGCCC GCATTGATAA AATCAAGGAC<br>TGGTTAGGAG ATGGCGTCAT TGCCGACTTC GACGACAAAC AGATCGAGCA AGCGCTGGCT<br>GATGTCGACG TCCCCATTGT TGGGGTTGGC GGCTCGTATC ACCTTGCAGA AAGTTACCCA<br>CCCGTTCATT ACATTGCCAC CGATAACTAT GCGCTGGTTG AAAGCGCATT TTTGCATTTA<br>AAAGAGAAAG GCGTTAACCG CTTTGCTTTT TATGGTCTTC CGGAATCAAG CGGCAAACGT<br>TGGGCCACTG AGCGCGAATA TGCATTTCGT CAGCTTGTCG CCGAAGAAAA GTATCGCGGA<br>GTGGTTTATC AGGGGTTAGA AACCGCGCCA GAGAACTGGC AACACGCGCA AAATCGGCTG<br>GCAGACTGGC TACAAACGCT ACCACCGCAA ACCGGGATTA TTGCCGTTAC TGACGCCCGA<br>GCGCGGCATA TTCTGCAAGT ATGTGAACAT CTACATATTC CCGTACCGGA AAAATTATGC<br>GTGATTGGCA TCGATAACGA AGAACTGACC CGCTATCTGT CGCGTGTCGC CCTTTCTTCG<br>GTCGCTCAGG GCGCGCGGCA AATGGGCTAT CAGGCGGCAA AACTGTTGCA TCGATTATTA<br>GATAAAGAAG AAATGCCGCT ACAGCGAATT TTGGTCCCAC CAGTTCGCGT CATTGAACGG<br>CGCTCAACAG ATTATCGCTC GCTGACCGAT CCCGCCGTTA TTCAGGCCAT GCATTACATT<br>CGTAATCACG CCTGTAAAGG GATTAAAGTG GATCAGGTAC TGGATGCGGT CGGGATCTCG<br>CGCTCCAATC TTGAGAAGCG TTTTAAAGAA GAGGTGGGTG AAACCATCCA TGCCATGATT<br>CATGCCGAGA AGCTGGAGAA AGCGCGCAGT CTGCTGATTT CAACCACCTT GTCGATCAAT<br>GAGATATCGC AAATGTGCGG TTATCCATCG CTGCAATATT TCTACTCTGT TTTTAAAAAA<br>GCATATGACA CGACGCCAAA AGAGTATCGC GATGTAAATA GCGAGGTCAT GTTG<font color=red>TAG</font>GCG<br>ATGATGAGAA TTGTCGGCGT CACATCAGGT AACGCTGCGT GGTTGTCGGA TGCGGCGTGA<br>ACGCCTTATC CGACCTACCC GCCAGGCATG ATAAAACGCA CCAACAACGC TTCAGGCTCG<br>TAGCTCAACT GCCTGAGACA AAGTAAAA<font color= | <html><pre>ATTTTGATAA AAATTTTCTC AAAGCCGGTT ACGTATTACC GGTTTTGAGT TTTTGCATGA<br>TTCAGCAGGA AAAGAACC<font color=green>a</font><font color=green>t</font> <font color=green>g</font>TTTACTAAA CGTCACCGCA TCACATTACT GTTCAATGCC<br>AATAAAGCCT ATGACCGGCA GGTAGTAGAA GGCGTAGGGG AATATTTACA GGCGTCACAA<br>TCGGAATGGG ATATTTTCAT TGAAGAAGAT TTCCGCGCCC GCATTGATAA AATCAAGGAC<br>TGGTTAGGAG ATGGCGTCAT TGCCGACTTC GACGACAAAC AGATCGAGCA AGCGCTGGCT<br>GATGTCGACG TCCCCATTGT TGGGGTTGGC GGCTCGTATC ACCTTGCAGA AAGTTACCCA<br>CCCGTTCATT ACATTGCCAC CGATAACTAT GCGCTGGTTG AAAGCGCATT TTTGCATTTA<br>AAAGAGAAAG GCGTTAACCG CTTTGCTTTT TATGGTCTTC CGGAATCAAG CGGCAAACGT<br>TGGGCCACTG AGCGCGAATA TGCATTTCGT CAGCTTGTCG CCGAAGAAAA GTATCGCGGA<br>GTGGTTTATC AGGGGTTAGA AACCGCGCCA GAGAACTGGC AACACGCGCA AAATCGGCTG<br>GCAGACTGGC TACAAACGCT ACCACCGCAA ACCGGGATTA TTGCCGTTAC TGACGCCCGA<br>GCGCGGCATA TTCTGCAAGT ATGTGAACAT CTACATATTC CCGTACCGGA AAAATTATGC<br>GTGATTGGCA TCGATAACGA AGAACTGACC CGCTATCTGT CGCGTGTCGC CCTTTCTTCG<br>GTCGCTCAGG GCGCGCGGCA AATGGGCTAT CAGGCGGCAA AACTGTTGCA TCGATTATTA<br>GATAAAGAAG AAATGCCGCT ACAGCGAATT TTGGTCCCAC CAGTTCGCGT CATTGAACGG<br>CGCTCAACAG ATTATCGCTC GCTGACCGAT CCCGCCGTTA TTCAGGCCAT GCATTACATT<br>CGTAATCACG CCTGTAAAGG GATTAAAGTG GATCAGGTAC TGGATGCGGT CGGGATCTCG<br>CGCTCCAATC TTGAGAAGCG TTTTAAAGAA GAGGTGGGTG AAACCATCCA TGCCATGATT<br>CATGCCGAGA AGCTGGAGAA AGCGCGCAGT CTGCTGATTT CAACCACCTT GTCGATCAAT<br>GAGATATCGC AAATGTGCGG TTATCCATCG CTGCAATATT TCTACTCTGT TTTTAAAAAA<br>GCATATGACA CGACGCCAAA AGAGTATCGC GATGTAAATA GCGAGGTCAT GTTG<font color=red>TAG</font>GCG<br>ATGATGAGAA TTGTCGGCGT CACATCAGGT AACGCTGCGT GGTTGTCGGA TGCGGCGTGA<br>ACGCCTTATC CGACCTACCC GCCAGGCATG ATAAAACGCA CCAACAACGC TTCAGGCTCG<br>TAGCTCAACT GCCTGAGACA AAGTAAAA<font color=blue>AG CCTTATCCGA CTGACAAGTC GGATAAGGCT</font><br>CAAGGAAATG CAAT</pre></html> | ||
<h3>Primers:</h3> | <h3>Primers:</h3> |
Revision as of 07:15, 12 June 2007
June 11th, 2007: 9:30-1:00, 3:45-5:00
XylR with promoter and rbs and terminator (right)
<html><pre>ATTTTGATAA AAATTTTCTC AAAGCCGGTT ACGTATTACC GGTTTTGAGT TTTTGCATGA<br>TTCAGCAGGA AAAGAACC<font color=green>a</font><font color=green>t</font> <font color=green>g</font>TTTACTAAA CGTCACCGCA TCACATTACT GTTCAATGCC<br>AATAAAGCCT ATGACCGGCA GGTAGTAGAA GGCGTAGGGG AATATTTACA GGCGTCACAA<br>TCGGAATGGG ATATTTTCAT TGAAGAAGAT TTCCGCGCCC GCATTGATAA AATCAAGGAC<br>TGGTTAGGAG ATGGCGTCAT TGCCGACTTC GACGACAAAC AGATCGAGCA AGCGCTGGCT<br>GATGTCGACG TCCCCATTGT TGGGGTTGGC GGCTCGTATC ACCTTGCAGA AAGTTACCCA<br>CCCGTTCATT ACATTGCCAC CGATAACTAT GCGCTGGTTG AAAGCGCATT TTTGCATTTA<br>AAAGAGAAAG GCGTTAACCG CTTTGCTTTT TATGGTCTTC CGGAATCAAG CGGCAAACGT<br>TGGGCCACTG AGCGCGAATA TGCATTTCGT CAGCTTGTCG CCGAAGAAAA GTATCGCGGA<br>GTGGTTTATC AGGGGTTAGA AACCGCGCCA GAGAACTGGC AACACGCGCA AAATCGGCTG<br>GCAGACTGGC TACAAACGCT ACCACCGCAA ACCGGGATTA TTGCCGTTAC TGACGCCCGA<br>GCGCGGCATA TTCTGCAAGT ATGTGAACAT CTACATATTC CCGTACCGGA AAAATTATGC<br>GTGATTGGCA TCGATAACGA AGAACTGACC CGCTATCTGT CGCGTGTCGC CCTTTCTTCG<br>GTCGCTCAGG GCGCGCGGCA AATGGGCTAT CAGGCGGCAA AACTGTTGCA TCGATTATTA<br>GATAAAGAAG AAATGCCGCT ACAGCGAATT TTGGTCCCAC CAGTTCGCGT CATTGAACGG<br>CGCTCAACAG ATTATCGCTC GCTGACCGAT CCCGCCGTTA TTCAGGCCAT GCATTACATT<br>CGTAATCACG CCTGTAAAGG GATTAAAGTG GATCAGGTAC TGGATGCGGT CGGGATCTCG<br>CGCTCCAATC TTGAGAAGCG TTTTAAAGAA GAGGTGGGTG AAACCATCCA TGCCATGATT<br>CATGCCGAGA AGCTGGAGAA AGCGCGCAGT CTGCTGATTT CAACCACCTT GTCGATCAAT<br>GAGATATCGC AAATGTGCGG TTATCCATCG CTGCAATATT TCTACTCTGT TTTTAAAAAA<br>GCATATGACA CGACGCCAAA AGAGTATCGC GATGTAAATA GCGAGGTCAT GTTG<font color=red>TAG</font>GCG<br>ATGATGAGAA TTGTCGGCGT CACATCAGGT AACGCTGCGT GGTTGTCGGA TGCGGCGTGA<br>ACGCCTTATC CGACCTACCC GCCAGGCATG ATAAAACGCA CCAACAACGC TTCAGGCTCG<br>TAGCTCAACT GCCTGAGACA AAGTAAAA<font color=blue>AG CCTTATCCGA CTGACAAGTC GGATAAGGCT</font><br>CAAGGAAATG CAAT</pre></html>
Primers:
gaattcgcggccgcttctagag TTTTGATAAA left side
ctgcagcggccgctactagta ATTGCATTTC right side
XylR with promoter and rbs (left)
<html><pre><font color=red>CTA</font>CAACATG ACCTCGCTAT TTACATCGCG ATACTCTTTT GGCGTCGTGT CATATGCTTT TTTAAAAACA GAGTAGAAAT ATTGCAGCGA TGGATAACCG CACATTTGCG ATATCTCATT GATCGACAAG GTGGTTGAAA TCAGCAGACT GCGCGCTTTC TCCAGCTTCT CGGCATGAAT CATGGCATGG ATGGTTTCAC CCACCTCTTC TTTAAAACGC TTCTCAAGAT TGGAGCGCGA GATCCCGACC GCATCCAGTA CCTGATCCAC TTTAATCCCT TTACAGGCGT GATTACGAAT GTAATGCATG GCCTGAATAA CGGCGGGATC GGTCAGCGAG CGATAATCTG TTGAGCGCCG TTCAATGACG CGAACTGGTG GGACCAAAAT TCGCTGTAGC GGCATTTCTT CTTTATCTAA TAATCGATGC AACAGTTTTG CCGCCTGATA GCCCATTTGC CGCGCGCCCT GAGCGACCGA AGAAAGGGCG ACACGCGACA GATAGCGGGT CAGTTCTTCG TTATCGATGC CAATCACGCA TAATTTTTCC GGTACGGGAA TATGTAGATG TTCACATACT TGCAGAATAT GCCGCGCTCG GGCGTCAGTA ACGGCAATAA TCCCGGTTTG CGGTGGTAGC GTTTGTAGCC AGTCTGCCAG CCGATTTTGC GCGTGTTGCC AGTTCTCTGG CGCGGTTTCT AACCCCTGAT AAACCACTCC GCGATACTTT TCTTCGGCGA CAAGCTGACG AAATGCATAT TCGCGCTCAG TGGCCCAACG TTTGCCGCTT GATTCCGGAA GACCATAAAA AGCAAAGCGG TTAACGCCTT TCTCTTTTAA ATGCAAAAAT GCGCTTTCAA CCAGCGCATA GTTATCGGTG GCAATGTAAT GAACGGGTGG GTAACTTTCT GCAAGGTGAT ACGAGCCGCC AACCCCAACA ATGGGGACGT CGACATCAGC CAGCGCTTGC TCGATCTGTT TGTCGTCGAA GTCGGCAATG ACGCCATCTC CTAACCAGTC CTTGATTTTA TCAATGCGGG CGCGGAAATC TTCTTCAATG AAAATATCCC ATTCCGATTG TGACGCCTGT AAATATTCCC CTACGCCTTC TACTACCTGC CGGTCATAGG CTTTATTGGC ATTGAACAGT AATGTGATGC GGTGACGTTT AGTAAACATG GTTCTTTTCC TGCTGAAT<font color=green>ca t</font>GCAAAAACT CAAAACCGGT AATACGTAAC CGGCTTTGAG AAAATTTTTA TCAAAAT</pre></html>
Primers:
gaattcgcggccgcttctagag CTACAACATG left side
ctgcagcggccgctactagta TTTTGATAAA right side
XylR open reading frame (right)
<html><pre><font color=green>atg</font>TTTACTA AACGTCACCG CATCACATTA CTGTTCAATG CCAATAAAGC CTATGACCGG CAGGTAGTAG AAGGCGTAGG GGAATATTTA CAGGCGTCAC AATCGGAATG GGATATTTTC ATTGAAGAAG ATTTCCGCGC CCGCATTGAT AAAATCAAGG ACTGGTTAGG AGATGGCGTC ATTGCCGACT TCGACGACAA ACAGATCGAG CAAGCGCTGG CTGATGTCGA CGTCCCCATT GTTGGGGTTG GCGGCTCGTA TCACCTTGCA GAAAGTTACC CACCCGTTCA TTACATTGCC ACCGATAACT ATGCGCTGGT TGAAAGCGCA TTTTTGCATT TAAAAGAGAA AGGCGTTAAC CGCTTTGCTT TTTATGGTCT TCCGGAATCA AGCGGCAAAC GTTGGGCCAC TGAGCGCGAA TATGCATTTC GTCAGCTTGT CGCCGAAGAA AAGTATCGCG GAGTGGTTTA TCAGGGGTTA GAAACCGCGC CAGAGAACTG GCAACACGCG CAAAATCGGC TGGCAGACTG GCTACAAACG CTACCACCGC AAACCGGGAT TATTGCCGTT ACTGACGCCC GAGCGCGGCA TATTCTGCAA GTATGTGAAC ATCTACATAT TCCCGTACCG GAAAAATTAT GCGTGATTGG CATCGATAAC GAAGAACTGA CCCGCTATCT GTCGCGTGTC GCCCTTTCTT CGGTCGCTCA GGGCGCGCGG CAAATGGGCT ATCAGGCGGC AAAACTGTTG CATCGATTAT TAGATAAAGA AGAAATGCCG CTACAGCGAA TTTTGGTCCC ACCAGTTCGC GTCATTGAAC GGCGCTCAAC AGATTATCGC TCGCTGACCG ATCCCGCCGT TATTCAGGCC ATGCATTACA TTCGTAATCA CGCCTGTAAA GGGATTAAAG TGGATCAGGT ACTGGATGCG GTCGGGATCT CGCGCTCCAA TCTTGAGAAG CGTTTTAAAG AAGAGGTGGG TGAAACCATC CATGCCATGA TTCATGCCGA GAAGCTGGAG AAAGCGCGCA GTCTGCTGAT TTCAACCACC TTGTCGATCA ATGAGATATC GCAAATGTGC GGTTATCCAT CGCTGCAATA TTTCTACTCT GTTTTTAAAA AAGCATATGA CACGACGCCA AAAGAGTATC GCGATGTAAA TAGCGAGGTC ATGTTG<font color=red>TAG</font></pre></html>
Primers:
gaattcgcggccgcttctagag ATGTTTACTA left side
ctgcagcggccgctactagta CTACAACATG right side
XylR open reading frame (left)
<html><pre><font color=red>CTA</font>CAACATG ACCTCGCTAT TTACATCGCG ATACTCTTTT GGCGTCGTGT CATATGCTTT TTTAAAAACA GAGTAGAAAT ATTGCAGCGA TGGATAACCG CACATTTGCG ATATCTCATT GATCGACAAG GTGGTTGAAA TCAGCAGACT GCGCGCTTTC TCCAGCTTCT CGGCATGAAT CATGGCATGG ATGGTTTCAC CCACCTCTTC TTTAAAACGC TTCTCAAGAT TGGAGCGCGA GATCCCGACC GCATCCAGTA CCTGATCCAC TTTAATCCCT TTACAGGCGT GATTACGAAT GTAATGCATG GCCTGAATAA CGGCGGGATC GGTCAGCGAG CGATAATCTG TTGAGCGCCG TTCAATGACG CGAACTGGTG GGACCAAAAT TCGCTGTAGC GGCATTTCTT CTTTATCTAA TAATCGATGC AACAGTTTTG CCGCCTGATA GCCCATTTGC CGCGCGCCCT GAGCGACCGA AGAAAGGGCG ACACGCGACA GATAGCGGGT CAGTTCTTCG TTATCGATGC CAATCACGCA TAATTTTTCC GGTACGGGAA TATGTAGATG TTCACATACT TGCAGAATAT GCCGCGCTCG GGCGTCAGTA ACGGCAATAA TCCCGGTTTG CGGTGGTAGC GTTTGTAGCC AGTCTGCCAG CCGATTTTGC GCGTGTTGCC AGTTCTCTGG CGCGGTTTCT AACCCCTGAT AAACCACTCC GCGATACTTT TCTTCGGCGA CAAGCTGACG AAATGCATAT TCGCGCTCAG TGGCCCAACG TTTGCCGCTT GATTCCGGAA GACCATAAAA AGCAAAGCGG TTAACGCCTT TCTCTTTTAA ATGCAAAAAT GCGCTTTCAA CCAGCGCATA GTTATCGGTG GCAATGTAAT GAACGGGTGG GTAACTTTCT GCAAGGTGAT ACGAGCCGCC AACCCCAACA ATGGGGACGT CGACATCAGC CAGCGCTTGC TCGATCTGTT TGTCGTCGAA GTCGGCAATG ACGCCATCTC CTAACCAGTC CTTGATTTTA TCAATGCGGG CGCGGAAATC TTCTTCAATG AAAATATCCC ATTCCGATTG TGACGCCTGT AAATATTCCC CTACGCCTTC TACTACCTGC CGGTCATAGG CTTTATTGGC ATTGAACAGT AATGTGATGC GGTGACGTTT AGTAAA<font color=green>cat</font></pre></html>
Primers:
gaattcgcggccgcttctagag CTACAACATG left side
ctgcagcggccgctactagta ATGTTTACTA right side