IGEM:PennState/Labbook/GalenLynch/2007-6-20: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
<h1>'''June 20th, 2007''': 9:45- | <h1>'''June 20th, 2007''': 9:45-5:00</h1> | ||
*Troubleshooting experiments | *Troubleshooting experiments | ||
**bizarre negative control on enzyme assays suggest that some restriction occured | **bizarre negative control on enzyme assays suggest that some restriction occured | ||
Latest revision as of 08:07, 21 June 2007
June 20th, 2007: 9:45-5:00
- Troubleshooting experiments
- bizarre negative control on enzyme assays suggest that some restriction occured
- all transformations failed
- Single unifying trait of expirements: amp
- Laboratory management
- Set up anhydrous containers for 100% ethanol and kanamycin
- Need to buy agarose for Dr.Richard's lab
- Began processing of switching to SYBR green instead of ethidium bromide
- SYBR is less mutagenic, may have better results, and would use dual purpose (GFP) filter
- Real time visualization
- No UV, less damage to DNA and safre to use
- Pre stain, less toxic, .3 cents per sample
- Lambda project
- Looked for part avaliability, explored lambda genome
