IGEM:PennState/Labbook/GalenLynch/2007-7-3

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Revision as of 08:56, 5 July 2007 by Tachyon60 (talk | contribs) (New page: <h1>'''July 3rd, 2007''':11:00-4:30</h1> *Sybr green stain from yesterday didn't work, set out on debugging the sybr green system **Bought materials, read proper dilution and storage techn...)
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July 3rd, 2007:11:00-4:30

  • Sybr green stain from yesterday didn't work, set out on debugging the sybr green system
    • Bought materials, read proper dilution and storage techniques
  • Decided to test the three different types of sybr-green stains avaliable
    • Post stain
    • Precast
    • Sample loading
  • Prepared post-stain for previously stained gels (ethidium bromide)
    • Made 10000x dilution into pH 8.0 TAE buffer (200mL)
    • Incubated for 40 min
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