IGEM:PennState/Labbook/GalenLynch/2007-9-4: Difference between revisions
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<h1>'''July 3rd, 2007''':11:00-?:??</h1> | <h1>'''July 3rd, 2007''':11:00-?:??</h1> | ||
*Sybr green stain from yesterday didn't work, set out on debugging the sybr green system | |||
**Bought materials, read proper dilution and storage techniques | |||
*Decided to test the three different types of sybr-green stains avaliable | |||
**Post stain | |||
**Precast | |||
**Sample loading | |||
*Prepared post-stain for previously stained gels (ethidium bromide) | |||
**Made 10000x dilution into pH 8.0 TAE buffer (200mL) | |||
**Incubated for 40 min | |||
Revision as of 13:04, 3 July 2007
July 3rd, 2007:11:00-?:??
- Sybr green stain from yesterday didn't work, set out on debugging the sybr green system
- Bought materials, read proper dilution and storage techniques
- Decided to test the three different types of sybr-green stains avaliable
- Post stain
- Precast
- Sample loading
- Prepared post-stain for previously stained gels (ethidium bromide)
- Made 10000x dilution into pH 8.0 TAE buffer (200mL)
- Incubated for 40 min
