IGEM:PennState/Labbook/GalenLynch/2007-9-4

July 3rd, 2007:11:00-?:??

• Sybr green stain from yesterday didn't work, set out on debugging the sybr green system
  • Bought materials, read proper dilution and storage techniques
• Decided to test the three different types of sybr-green stains avaliable
  • Post stain
  • Precast
  • Sample loading
• Prepared post-stain for previously stained gels (ethidium bromide)
  • Made 10000x dilution into pH 8.0 TAE buffer (200mL)
  • Incubated for 40 min