IGEM:PennState/Labbook/LucienWeiss
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Lucien Weiss Lab Notebook
- Penn State iGEM 2005-2007
- Penn State Undergraduate
- Email luw134@psu.edu
- Phone (814) 880 5909
March 12, 2007: Motility Assay
<body> Cell Notation:
- YFP Sender Cells-Sender (LVA+)-RP3087 w/ [I14032+B0030+C0161 A3]+E0430
- Sender Cells-Sender-RP3087 w/ I14032+B0030+C0161 (B2)
- Receiver-RFP+Receiver-RP3087 w/ I13321+[J09855+B0033+J09274]
Plate Notation
- 20EikenAgar-
- .25g Bactotryptone
- .20g NaCl
- .15g sodium citrate
- ^.05g Eiken Agar (for 20EikenAgar)
- ^.075g Eiken Agar (for 30EikenAgar)
- ^.10g Eiken Agar (for 40EikenAgar
- ^choose one
note: 20EikenAgar refers to .20%
Picture information Pictures taken at 8 hours after cells first plated. on round plates: Sender cell colony (on left hand side for round plates) Receiver Cells placed at a variety of distances away on square plates: sender cell colonies (top row going across) Receiver cells (diagonally downward)
Experiment Procedure Summary Plasmids transformed into RP3087 Grown overnight at 37 degrees on Kan plate Starter culture grown overnight in 3mL LB+Kan at 30 degrees diluted 1:100 and grown in 3mL LB+Kan at 30 degrees until OD ~.45 3uL placed on motility plates Pictures taken at 8 hours Additional Pictures taken at 20 hours</body>
March 29-April 1, 2007: Institute of Biological Engineering
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Poster: Bacterial Relay Race |
March 30, 2007: Motility Assay
April 22, 2007: Project Proposal Presentations
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Project Proposal: Pressure Sensing in Bacteria       
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Project Proposal: Bi and Sept Stable Oscillator      
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