IGEM:PennState/Labbook/Nimrah Ahmed

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (Circuit project ideas:)
(Expanding the color changer idea)
Line 2: Line 2:
===Circuit project ideas:===
===Circuit project ideas:===
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
-
*'''Sequential cell color change'''
+
====Sequential cell color change====
-
**ex. Green->Red->Yellow->repeat.
+
*ex. Green->Red->Yellow->repeat.
-
**System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
+
*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
-
**Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
+
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
-
**Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
+
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
-
**Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
+
 
 +
=====Components=====
 +
* Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
 +
* Promoter: lac, responds to organic
 +
* Color proteins
 +
 
 +
Options:
 +
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

Revision as of 11:20, 15 May 2008

Contents

Meeting 5/15/2008

Circuit project ideas:

Sequential cell color change

  • ex. Green->Red->Yellow->repeat.
  • System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
  • Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
  • Rather than shake, stir by thermal convection or a magnetic stirrer
Components
  • Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
  • Promoter: lac, responds to organic
  • Color proteins

Options:

  • Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...

(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

Personal tools