IGEM:PennState/Labbook/Nimrah Ahmed
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===Circuit project ideas:=== | ===Circuit project ideas:=== | ||
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf | * Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf | ||
| - | + | ====Sequential cell color change==== | |
| - | + | *ex. Green->Red->Yellow->repeat. | |
| - | + | *System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change. | |
| - | + | *Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter | |
| - | + | *Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer''' | |
| - | **Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins... | + | |
| + | =====Components===== | ||
| + | * Organic compound: hydrophobic, heavy (won't evaporate), activates promoter | ||
| + | * Promoter: lac, responds to organic | ||
| + | * Color proteins | ||
| + | |||
| + | Options: | ||
| + | *Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins... | ||
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?) | (Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?) | ||
Revision as of 12:20, 15 May 2008
Contents |
Meeting 5/15/2008
Circuit project ideas:
- Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
Sequential cell color change
- ex. Green->Red->Yellow->repeat.
- System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
- Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
- Rather than shake, stir by thermal convection or a magnetic stirrer
Components
- Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
- Promoter: lac, responds to organic
- Color proteins
Options:
- Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
