IGEM:PennState/Labbook/Nimrah Ahmed: Difference between revisions

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(Expanding the color changer idea)
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===Circuit project ideas:===
===Circuit project ideas:===
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
* Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
*'''Sequential cell color change'''
====Sequential cell color change====
**ex. Green->Red->Yellow->repeat.
*ex. Green->Red->Yellow->repeat.
**System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
**Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
**Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
**Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
 
=====Components=====
* Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
* Promoter: lac, responds to organic
* Color proteins
 
Options:
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

Revision as of 09:20, 15 May 2008

Meeting 5/15/2008

Circuit project ideas:

Sequential cell color change

  • ex. Green->Red->Yellow->repeat.
  • System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
  • Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
  • Rather than shake, stir by thermal convection or a magnetic stirrer
Components
  • Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
  • Promoter: lac, responds to organic
  • Color proteins

Options:

  • Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...

(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

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