IGEM:PennState/Labbook/Nimrah Ahmed

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(Expanding the color changer idea)
m
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*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
*System: organic layer & bacterial aquatic layer in liquid form.  Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
-
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
 
=====Components=====
=====Components=====
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* Promoter: lac, responds to organic
* Promoter: lac, responds to organic
* Color proteins
* Color proteins
 +
 +
=====Circuit=====
 +
O -> A
 +
A+B -> C, GFP
 +
A+C -> B, YFP
 +
*Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
 +
*Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
Options:
Options:
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
 +
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
 +
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

Revision as of 12:23, 15 May 2008

Contents

Meeting 5/15/2008

Circuit project ideas:

Sequential cell color change

  • ex. Green->Red->Yellow->repeat.
  • System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
  • Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
Components
  • Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
  • Promoter: lac, responds to organic
  • Color proteins
Circuit

O -> A A+B -> C, GFP A+C -> B, YFP

  • Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
  • Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.

Options:

  • Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
  • Rather than shake, stir by thermal convection or a magnetic stirrer

(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)

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