IGEM:PennState/Labbook/Nimrah Ahmed: Difference between revisions
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*System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change. | *System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change. | ||
*Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter | *Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter | ||
=====Components===== | =====Components===== | ||
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* Promoter: lac, responds to organic | * Promoter: lac, responds to organic | ||
* Color proteins | * Color proteins | ||
=====Circuit===== | |||
O -> A | |||
A+B -> C, GFP | |||
A+C -> B, YFP | |||
*Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C. | |||
*Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc. | |||
Options: | Options: | ||
*Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins... | *Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins... | ||
*Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer''' | |||
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?) | (Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?) | ||
Revision as of 09:23, 15 May 2008
Meeting 5/15/2008
Circuit project ideas:
- Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
Sequential cell color change
- ex. Green->Red->Yellow->repeat.
- System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
- Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
Components
- Organic compound: hydrophobic, heavy (won't evaporate), activates promoter
- Promoter: lac, responds to organic
- Color proteins
Circuit
O -> A A+B -> C, GFP A+C -> B, YFP
- Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
- Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
Options:
- Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
- Rather than shake, stir by thermal convection or a magnetic stirrer
(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
