IGEM:PennState/Labbook/Nimrah Ahmed

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|colspan="2" style="background-color: #F2F2F2;" align="right"|[[/Entry_Base|Customize your entry pages]] [[Help:Notebook/Project_Base/Customize_entry_page|<html><img src="/images/a/aa/Help.png" border="0" /></html>]]
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==Description/Abstract==
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* Place short description of project or notes regarding this project
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==Notes==
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* Team meetings: Monday 5:00pm
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==Meeting 5/15/2008==
==Meeting 5/15/2008==
===Circuit project ideas:===
===Circuit project ideas:===
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[[User:Nimrah Ahmed|Nimrah Ahmed]] 12:26, 15 May 2008 (EDT)
[[User:Nimrah Ahmed|Nimrah Ahmed]] 12:26, 15 May 2008 (EDT)
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==Work Day Monday 5/19/2008==
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*Introduction to lab & equipment
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===Making Media===

Revision as of 10:22, 19 May 2008

Search this Project

Customize your entry pages

Description/Abstract

  • Place short description of project or notes regarding this project


Notes

  • Team meetings: Monday 5:00pm

Recent changes




Meeting 5/15/2008

Circuit project ideas:

Sequential cell color change

  • ex. Green->Red->Yellow->repeat.
  • System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
  • Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
Components
  • Organic compound: hydrophobic, heavy (won't evaporate), is recognized by cell
  • Recognition compound(s): recognize organic compound and synthesize/activate something that activates promoter
  • Promoter: probably lac
  • Color proteins
Circuit

O -> A

A+B -> C, GFP

A+C -> B, YFP


  • Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
  • Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
Options:
  • Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
  • Rather than shake, stir by thermal convection or a magnetic stirrer

(Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)


Nimrah Ahmed 12:26, 15 May 2008 (EDT)


Work Day Monday 5/19/2008

  • Introduction to lab & equipment

Making Media

Personal tools