< IGEM:PennState(Difference between revisions)
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| | ==Description/Abstract== | | ==Description/Abstract== |
| - | * Place short description of project or notes regarding this project
| + | Currently working on finding a project |
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| - | ==Meeting 5/15/2008==
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| - | ===Circuit project ideas:===
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| - | * Genetic Circuit basics: http://www.princeton.edu/~rweiss/bio-programming/weiss-natural-computing-2003.pdf
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| - | ====Sequential cell color change====
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| - | *ex. Green->Red->Yellow->repeat.
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| - | *System: organic layer & bacterial aquatic layer in liquid form. Shake to combine organic layer w/ bacterial aquatic layer -> initiate color change.
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| - | *Structure: Messenger protein downstream of color protein, downstream of organic molecule-activated promoter
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| - | =====Components=====
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| - | * Organic compound: hydrophobic, heavy (won't evaporate), is recognized by cell
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| - | * Recognition compound(s): recognize organic compound and synthesize/activate something that activates promoter
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| - | * Promoter: probably lac
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| - | * Color proteins
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| - | =====Circuit=====
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| - | O -> A
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| - | A+B -> C, GFP
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| - | A+C -> B, YFP
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| - | *Degradation tags on A, B, C, GFP, YFP, strongest on A, weakest on B and C.
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| - | *Intended result: shake once: GFP, shake again: YFP, shake again, GFP, etc.
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| - | =====Options:=====
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| - | *Bioluminescense : proteins like "M-Cherry" instead of flourescent proteins...
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| - | *Rather than shake, stir by '''thermal convection''' or a '''magnetic stirrer'''
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| - | (Peking U did something like this--sequential color change--but UV activated. MIT team idea: bacterial lava lamp?)
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| - | [[User:Nimrah Ahmed|Nimrah Ahmed]] 12:26, 15 May 2008 (EDT)
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| - | ==Work Day Monday 5/19/2008==
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| - | *Introduction to lab & equipment
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| - | ===Making Media===
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