IGEM:PennState/Labbook/NoahJohnson

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Line 33: Line 33:
#*0.5 EcoR1
#*0.5 EcoR1
#*0.5 SpeI
#*0.5 SpeI
 +
#*
#Vector A,B,C
#Vector A,B,C
#*3.5 H20
#*3.5 H20
Line 40: Line 41:
#*0.5 EcoR1
#*0.5 EcoR1
#*0.5 XbaI
#*0.5 XbaI
 +
#*
#Vector -
#Vector -
#*6.5 H2O
#*6.5 H2O
#*3.5 DNA (J31004: A (LW0003 LW-1))
#*3.5 DNA (J31004: A (LW0003 LW-1))
 +
#*
#EcoR1 Vector
#EcoR1 Vector
#*4 H2O
#*4 H2O
Line 49: Line 52:
#*1 BSA
#*1 BSA
#*0.5 EcoR1
#*0.5 EcoR1
 +
#*
#XbaI Vector
#XbaI Vector
#*4 H2O
#*4 H2O
Line 55: Line 59:
#*1 BSA
#*1 BSA
#*0.5 Xba
#*0.5 Xba
 +
#*
#SpeI Vector
#SpeI Vector
#*4 H2O
#*4 H2O

Revision as of 13:56, 24 May 2007

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5-24-07

Restriction Digest

  1. Insert A,B,C
    • 3.5 H20
    • 3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 SpeI
  2. Vector A,B,C
    • 3.5 H20
    • 3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 XbaI
  3. Vector -
    • 6.5 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
  4. EcoR1 Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
  5. XbaI Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 Buffer 2
    • 1 BSA
    • 0.5 Xba
  6. SpeI Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 Buffer 2
    • 1 BSA
    • 0.5 SpeI
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