IGEM:PennState/Labbook/NoahJohnson: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
 
(35 intermediate revisions by 3 users not shown)
Line 1: Line 1:
<h1 style="color: #AAAAAA">'''[[User:nrj5011|Noah Johnson]] Lab Notebook'''</h1>
<h1>[[user:nrj5011|Noah Johnson]]</h1>
 
*Penn State iGEM 2007
*Penn State iGEM 2007
**[[http://openwetware.org/wiki/IGEM:PennState iGEM 2007]]
**[[http://openwetware.org/wiki/IGEM:PennState iGEM 2007]]
*Penn State Undergraduate Mathematics and Biological Engineering
*Penn State Undergraduate - BioEngineering
*Email [mailto:nrj5011@psu.edu nrj5011@psu.edu]
*Email [mailto:nrj5011@psu.edu nrj5011@psu.edu]
*Phone (814)574 2108
*Phone (814) 441 2037
 
<h2>'''Calender'''</h2>
<calendar>
name=IGEM:PennState/Labbook/NoahJohnson
date=2007/04/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
<calendar>
name=IGEM:PennState/Labbook/NoahJohnson
date=2007/07/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
<calendar>
name=IGEM:PennState/Labbook/NoahJohnson
date=2007/10/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
<calendar>
name=IGEM:PennState/Labbook/NoahJohnson
date=2008/01/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
<calendar>
name=IGEM:PennState/Labbook/NoahJohnson
date=2008/04/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
<h2>[<span class="_togglegroup _toggle _toggler">April 30, 2007-Bacterial Conjugation Presentation</span><span class="_toggle _toggler" style="display:none;">April 30, 2007</span>]</h2>
<div class="_toggle" style="display:none;">
[<span class="_togglegroup _toggle _toggler">Project Proposal: Bacterial Conjugation</span><span class="_toggle _toggler" style="display:none;">Close Project Proposal: Bacterial Conjugation</span>]
<div class="_toggle" style="display:none;">
 
<table|left>
 
<tr>
  <td>[[Image:psuigemnrj5011conjugation1.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011conjugation2.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011conjugation3.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011conjugation4.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011conjugation5.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011conjugation6.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011conjugation7.jpg|thumb|]]</td>
  </tr>
</table>
</div>
</div>
 
<h2>[<span class="_togglegroup _toggle _toggler">May 24, 2007-Restriction Digest</span><span class="_toggle _toggler" style="display:none;">May 24, 2007</span>]</h2>
<div class="_toggle" style="display:none;">
[<span class="_togglegroup _toggle _toggler">Restriction Digest</span><span class="_toggle _toggler" style="display:none;">Close Restriction Digest</span>]
<div class="_toggle" style="display:none;">
 
#Insert A,B,C
#*3.5 H20
#*3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
#*1 EcoR1 Buf
#*1 BSA
#*0.5 EcoR1
#*0.5 SpeI
#Vector A,B,C
#*3.5 H20
#*3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
#*1 EcoR1 Buf(!WRONG:Should have been Buffer 2!)
#*1 BSA
#*0.5 EcoR1
#*0.5 XbaI
#Vector -
#*6.5 H2O
#*3.5 DNA (J31004: A (LW0003 LW-1))
#EcoR1 Vector
#*4 H2O
#*3.5 DNA (J31004: A (LW0003 LW-1))
#*1 EcoR1 Buf
#*1 BSA
#*0.5 EcoR1
#XbaI Vector
#*4 H2O
#*3.5 DNA (J31004: A (LW0003 LW-1))
#*1 Buffer 2
#*1 BSA
#*0.5 Xba
#SpeI Vector
#*4 H2O
#*3.5 DNA (J31004: A (LW0003 LW-1))
#*1 Buffer 2
#*1 BSA
#*0.5 SpeI
 
</div>
</div>
 
<h2>[<span class="_togglegroup _toggle _toggler">May 30, 2007-Biofilms Presentation</span><span class="_toggle _toggler" style="display:none;">May 30, 2007</span>]</h2>
<div class="_toggle" style="display:none;">
[<span class="_togglegroup _toggle _toggler">Project Proposal: Biofilms</span><span class="_toggle _toggler" style="display:none;">Close Project Proposal: Biofilms</span>]
<div class="_toggle" style="display:none;">
 
<table|left>
 
<tr>
  <td>[[Image:psuigemnrj5011biofilms1.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011biofilms2.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011biofilms3.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011biofilms4.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011biofilms5.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011biofilms6.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011biofilms7.jpg|thumb|]]</td>
  </tr>
</table>
 
</div>
</div>
 
<h2>[<span class="_togglegroup _toggle _toggler">June 4, 2007-iGEM Meeting Presentation</span><span class="_toggle _toggler" style="display:none;">June 4, 2007</span>]</h2>
<div class="_toggle" style="display:none;">
[<span class="_togglegroup _toggle _toggler">iGEM Meeting Presentation</span><span class="_toggle _toggler" style="display:none;">iGEM Meeting Presentation</span>]
<div class="_toggle" style="display:none;">
 
<table|left>
 
<tr>
  <td>[[Image:psuigemnrj5011june041.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011june042.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011june043.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011june044.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011june045.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011june046.jpg|thumb|]]</td>
</tr>
<tr>
  <td>[[Image:psuigemnrj5011june047.jpg|thumb|]]</td>
  <td>[[Image:psuigemnrj5011june048.jpg|thumb|]]</td>
  </tr>
</table>
 
</div>
</div>
 
<h2>[<span class="_togglegroup _toggle _toggler">June 11, 2007-Primer Sequencing</span><span class="_toggle _toggler" style="display:none;">June 11, 2007</span>]</h2>
<div class="_toggle" style="display:none;">
[<span class="_togglegroup _toggle _toggler">XylA,B Primer</span><span class="_toggle _toggler" style="display:none;">XylA,B Primer</span>]
<div class="_toggle" style="display:none;">
 
3725940-3728788 Found on [http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11074 Ecocyc]
 
<html><pre>
TTACGCCATT AATGGCAGAA GTTGCTGATA GAGGCGACGG AACGTTTCTC GTCGTGGCTG
ATAAGCGGCA TAACGCTGCG CATCTGGTAG ATGCGACTGT TCTAACGGTA GTTGCGGCAA
CAATTCAATG AGCGATTTCT CTGGATTCGC CGCGATCTGC GCCAGCCTTG CTGCGCCCAG
TGCTGGCCCC ACATCCCCCC CCGTACGGTA ATCGAGCTGC TGACCGCTGA TATCCGCCAG
CATCTGACGC CAGTACTCAC TACGCGCCCC GCCCCCAATC AACGTAACAC TTTGCGGTTT
AATACCGCAG GCATGCACGA CATCCATGCC ATCTGCCAGC GCATAACCCA CGCCTTCCAG
CACTGCTCGC GCCAGTTCAT TGGGGCCATG TTGATGAGTC AAACCAAAGA AAACCCCCTT
CGCCTGGGGA TTATTGTGTG GCGTACGCTC GCCGGAAAGA TAAGGCAGAA ACCAAACTGG
CTCGGCACTT TCATCAGCCT GTTGAGCTGC AGCGATTAAA GCTGGGACAT TGCTCAGGCC
GGTTAATTTC GCGGCCCAAT CCAGACACGA CGCTGCACTC AGCATCACAG ACATTAAATG
CCAACGTTGC GGTAGCGCAT GGCAAAAGCT ATGTACGGCG CTTTCTGGCT TGCTTAAGAA
CCCTTCGCTG ACAGCAAAAT AGACCCCCGA CGTCCCCAGC GATAACATTG CCTGATTAGC
ATCAACCATT CCCACACCAA CTGCACCAGC TGCATTGTCG CCACCGCCTG CGACAACTGG
CACCGTCGCC ATACCCCACG CTTTCGCAAC TTCAGGTAAC AAAGCACCAG TAATTTCGCT
GCCTTCGTAT AATGCGGGCA TCTGGTCACG AGATAAGTCG CAAGCCTGCA GCATGACGTC
ACTCCAGTCA CGCTTTGCGA CATCCAGCCA CATGGTGCCA GCTGCGTCAG ACATATCGCT
GGCAAACTCC CCCGTCATAC GCAGACGCAA GTAATCTTTC GGTAATAATA CTTTGTCGAT
TTGACGGAAT ATCTCCGGCT CATGCCGCTG AACCCATAGC AATTTAGGCG CAGTAAATCC
GGGCATCATC AGGTTGCCGG TAATCACCCG CGATTGCGGA ACTCGCGCTT CCAGCAAAGT
GCACTCTTGC GCACAGCGCC CGTCGTTCCA CAAAATGGCA GGGCGTAACA CCCGTTGCTG
AGCATCCAGC AAGGTTGCTC CGTGCATCTG GCCGGCAATA CCCAATGCTT TAACGTCCTG
CAGAGAATGC TGATCGCCCA GAGCTTTCAT TGCGCGATCA GTTGCCTGCC ACCACTGTTC
CGGGTCTTGT TCCGACCAGA GTGGATGCGG GCGCGAAACG GTCAGCTTTT CCGTTTGCGC
AGCAACCACC TCACCCTGCT CGTTGAGCAA AATAACTTTT ACGCCCGAGG TGCCAAGATC
TATCCCGATA TACATATCGA TCGTTCCTTA AAAAAATGCC CGGTATCGCT ACCGATAACC
GGGCCAACGG ACTGCACAGT TAGCCGTTAT TTGTCGAACA GATAATGGTT TACCAGATTT
TCCAGTTGTT CCTGGCGACC ACTCTGATGC ACCGGAGACA AATGATGTTC CTGAGCATAT
TTGGCTAAAT CTGCCAGTGA CATTTGGCCT TTCAGGATTT GCTGGCCCAA TTCGCTATTC
CAGCCGGAAT AACGCTGCGC GATGCGTTTA TCCAGCTCGC CATCTTCAAT CATGCGCGCT
GCAATTTTCA GCGCCAGTGC CATCGTATCC ATCGCGCCGA TATGACCGTA AAACAGATCA
TATTTATCAG TACTTTGACG ACGTACTTTG GCATCGAAGT TCAGACCACC GGTGGTGAAA
CCGCCTGCTT TGAGAATTTC ATACATCACC AGCGCATTCT CTTCCACACT GTTCGGGAAC
TGGTCGGTGT CCCAGCCCAG TTGCGCATCG CCACGGTTGG CGTCGACAGA ACCGAACAGG
CCAAGCGCAA TGGCGGTGGC TATTTCATGA TGGAAAGAGT GACCTGCCAG CGTCGCGTGG
TTAGCTTCAA TGTTCAGTTT AATCTCTTTT TCCAGACCAA ACTGTTTCAG GAAGCCATAG
ACCGTCGCGG CATCGTAATC ATATTGATGT TTGGTCGGTT CTTGCGGTTT CGGTTCGATA
AGCAACGTGC CCTGGAAACC GATTTTATGT TTATGCTCAA CCACCATCTG CATAAAGCGG
CCCAGTTGTT CACGCTCCTG ACGCAAGTCG GTATTTAACA GCGTTTCGTA ACCTTCACGA
CCGCCCCACA GGACATAGTT TTCACCGCCC AATTTATGGG TTGCTTCCAT CGCTGTAACA
ACTTGCGTTG CCGCCCAGCT GAAGACTTCA GGATCTGGGT TCGTCGCCGC ACCCGCGCCG
TAGCGAGGGT TTGTAAAGCA GTTGGCCGTT CCCCACAGCA GCTTCACGCC GCTCTCTTCT
TGCTTGCCTG CCAGGACATC AACCATTTGC GCAAAATTAT TGATGTACTC TTTTAACGAC
GCGCCCTCAG GGGAAACATC CACATCGTGG AAGCAATAAA ATGGCACATG TAACTTGTGG
AAAAACTCAA ATGCGACATC TGCTTTACGC TTCGCCAACG CCAGTGCCTC ACCAGGCTGC
TGCCACGGAC GATTAAACGC CCCCACACCA AACATATCCG CCCCGTTCCA GCAGAAGGTG
TGCCAGTAGC AGGCGGCAAA ACGCAAGTGC TCTTCCATAC GCTTACCCAA CACCAGTTCG
TCGGGATTGT AGTGACGGAA TGCTAACGGG TTTGAGGATT TTGAGCCTTC ATAACGAACG
CGATCGAGCT GGTCAAAATA GGCTTGCAT</pre></html>
 
Left side primer:  gaattcgcggccgcttctagag TTACGCCATT AATGGCAGAA
 
Right side primer:  ctgcagcggccgctactagta ATGCAAGCCT ATTTTGACCA
 
</div>
 
[<span class="_togglegroup _toggle _toggler">XylR Promoter Region Primer</span><span class="_toggle _toggler" style="display:none;">XylR Promoter Region Primer</span>]
<div class="_toggle" style="display:none;">
 
 
3732905-3733001 Found on [http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG20253 Ecocyc]
 
<html><pre>CAACCAAACG CCGTTCTTGA TTTTGATAAA AATTTTCTCA AAGCCGGTTA CGTATTACCG
GTTTTGAGTT TTTGCATGAT TCAGCAGGAA AAGAACC</pre></html>
 
Left side primer:  gaattcgcggccgcttctagag CAACCAAACG CCGTTCTTGA


Right side primer:  ctgcagcggccgctactagta GGTTCTTTTC CTGCTGAATC


<h2>'''April 22, 2007: Project Proposal Presentations'''</h2>
</div>
</div>

Latest revision as of 12:50, 15 April 2008

Noah Johnson

Calender

<calendar> name=IGEM:PennState/Labbook/NoahJohnson date=2007/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:PennState/Labbook/NoahJohnson date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:PennState/Labbook/NoahJohnson date=2007/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:PennState/Labbook/NoahJohnson date=2008/01/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:PennState/Labbook/NoahJohnson date=2008/04/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

[April 30, 2007-Bacterial Conjugation Presentation]

[May 24, 2007-Restriction Digest]

[May 30, 2007-Biofilms Presentation]

[June 4, 2007-iGEM Meeting Presentation]

[June 11, 2007-Primer Sequencing]

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:PennState/Labbook/NoahJohnson&oldid=196860"