IGEM:PennState/Labbook/NoahJohnson/2007-5-24: Difference between revisions
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(New page: <h2>5-24-07</h2> '''Restriction Digest''' #Insert A,B,C #*3.5 H20 #*3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1)) #*1 EcoR1 Buf #*1 BSA #*0.5 EcoR1 #*0.5 SpeI #* #Vect...) |
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<h2> | <h2>May 24, 2007</h2> | ||
'''Restriction Digest''' | '''Restriction Digest''' | ||
| Line 9: | Line 9: | ||
#*0.5 EcoR1 | #*0.5 EcoR1 | ||
#*0.5 SpeI | #*0.5 SpeI | ||
#Vector A,B,C | #Vector A,B,C | ||
#*3.5 H20 | #*3.5 H20 | ||
#*3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1)) | #*3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1)) | ||
#*1 EcoR1 Buf | #*1 EcoR1 Buf(!WRONG:Should have been Buffer 2!) | ||
#*1 BSA | #*1 BSA | ||
#*0.5 EcoR1 | #*0.5 EcoR1 | ||
#*0.5 XbaI | #*0.5 XbaI | ||
#Vector - | #Vector - | ||
#*6.5 H2O | #*6.5 H2O | ||
#*3.5 DNA (J31004: A (LW0003 LW-1)) | #*3.5 DNA (J31004: A (LW0003 LW-1)) | ||
#EcoR1 Vector | #EcoR1 Vector | ||
#*4 H2O | #*4 H2O | ||
| Line 28: | Line 25: | ||
#*1 BSA | #*1 BSA | ||
#*0.5 EcoR1 | #*0.5 EcoR1 | ||
#XbaI Vector | #XbaI Vector | ||
#*4 H2O | #*4 H2O | ||
| Line 35: | Line 31: | ||
#*1 BSA | #*1 BSA | ||
#*0.5 Xba | #*0.5 Xba | ||
#SpeI Vector | #SpeI Vector | ||
#*4 H2O | #*4 H2O | ||
Latest revision as of 11:39, 30 May 2007
May 24, 2007
Restriction Digest
- Insert A,B,C
- 3.5 H20
- 3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
- 1 EcoR1 Buf
- 1 BSA
- 0.5 EcoR1
- 0.5 SpeI
- Vector A,B,C
- 3.5 H20
- 3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
- 1 EcoR1 Buf(!WRONG:Should have been Buffer 2!)
- 1 BSA
- 0.5 EcoR1
- 0.5 XbaI
- Vector -
- 6.5 H2O
- 3.5 DNA (J31004: A (LW0003 LW-1))
- EcoR1 Vector
- 4 H2O
- 3.5 DNA (J31004: A (LW0003 LW-1))
- 1 EcoR1 Buf
- 1 BSA
- 0.5 EcoR1
- XbaI Vector
- 4 H2O
- 3.5 DNA (J31004: A (LW0003 LW-1))
- 1 Buffer 2
- 1 BSA
- 0.5 Xba
- SpeI Vector
- 4 H2O
- 3.5 DNA (J31004: A (LW0003 LW-1))
- 1 Buffer 2
- 1 BSA
- 0.5 SpeI
