IGEM:PennState/Labbook/NoahJohnson/2007-5-24

5-24-07

Restriction Digest

1. Insert A,B,C
   • 3.5 H20
   • 3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
   • 1 EcoR1 Buf
   • 1 BSA
   • 0.5 EcoR1
   • 0.5 SpeI
2. Vector A,B,C
   • 3.5 H20
   • 3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
   • 1 EcoR1 Buf(!WRONG:Should have been Buffer 2!)
   • 1 BSA
   • 0.5 EcoR1
   • 0.5 XbaI
3. Vector -
   • 6.5 H2O
   • 3.5 DNA (J31004: A (LW0003 LW-1))
4. EcoR1 Vector
   • 4 H2O
   • 3.5 DNA (J31004: A (LW0003 LW-1))
   • 1 EcoR1 Buf
   • 1 BSA
   • 0.5 EcoR1
5. XbaI Vector
   • 4 H2O
   • 3.5 DNA (J31004: A (LW0003 LW-1))
   • 1 Buffer 2
   • 1 BSA
   • 0.5 Xba
6. SpeI Vector
   • 4 H2O
   • 3.5 DNA (J31004: A (LW0003 LW-1))
   • 1 Buffer 2
   • 1 BSA
   • 0.5 SpeI