IGEM:PennState/Labbook/NoahJohnson/2007-5-24

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May 24, 2007

Restriction Digest

  1. Insert A,B,C
    • 3.5 H20
    • 3.5 DNA (J23038: A (LW0006 LW-1),B (LW0007 LW-1),C (LW0008 LW-1))
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 SpeI
  2. Vector A,B,C
    • 3.5 H20
    • 3.5 DNA (J31004: A (LW0003 LW-1),B (LW0004 LW-1),C (LW0005 LW-1))
    • 1 EcoR1 Buf(!WRONG:Should have been Buffer 2!)
    • 1 BSA
    • 0.5 EcoR1
    • 0.5 XbaI
  3. Vector -
    • 6.5 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
  4. EcoR1 Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 EcoR1 Buf
    • 1 BSA
    • 0.5 EcoR1
  5. XbaI Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 Buffer 2
    • 1 BSA
    • 0.5 Xba
  6. SpeI Vector
    • 4 H2O
    • 3.5 DNA (J31004: A (LW0003 LW-1))
    • 1 Buffer 2
    • 1 BSA
    • 0.5 SpeI
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